Electrolyzed Hydrogen Water Protects against Ethanol-Induced Cytotoxicity by Regulating Aldehyde Metabolism-Associated Enzymes in the Hepatic Cell Line HepG2.
Electrolyzed Hydrogen Water Protects against Ethanol-Induced Cytotoxicity by Regulating Aldehyde Metabolism-Associated Enzymes in the Hepatic Cell Line HepG2.
复制标题
DOI：
10.3390/antiox10050801
复制
发表时间：
2021-05-19
期刊：
影响因子：
--
通讯作者：
Hara T
中科院分区：
文献类型：
--
作者：
Yano S;Wang J;Kabayama S;Hara T
过量饮酒会导致多系统疾病。其中，酒精性肝病是最常见、最严重的疾病。电解氢水（EHW）是水电解过程中在阴极产生的，含有大量的氢分子和少量具有碱性的铂纳米颗粒。在这项研究中，我们发现 EHW 通过减少肝细胞系 HepG2 细胞内乙醛（乙醇降解产生的有毒物质）来抑制乙醇诱导的细胞毒性。作用机制分析表明，EHW通过抑制乙醇脱氢酶来抑制乙醇向乙醛的代谢。 EHW还通过激活醛脱氢酶促进乙醛代谢为乙酸，从而降低HepG2细胞中的醛毒性和细胞内活性氧。这些功能与 EHW 中分子氢的浓度相关，并通过脱气处理消除，表明分子氢可能作为抑制乙醇诱导的肝细胞损伤的功能因子。此外，具有高溶解氢分子的氢水对乙醇表现出与EHW相同的肝细胞保护作用。这些结果表明 EHW 可能有助于预防酒精性肝病。
Excessive alcohol consumption can cause multi-systemic diseases. Among them, alcoholic liver disease is the most frequent and serious disease. Electrolytic hydrogen water (EHW) is produced at the cathode during electrolysis of water and contains a large amount of molecular hydrogen and a low content of platinum nanoparticles with alkaline properties. In this study, we found that EHW inhibits ethanol-induced cytotoxicity by decreasing the intracellular acetaldehyde, a toxic substance produced by ethanol degradation, in hepatocyte cell lines HepG2. Analysis of the mechanism of action revealed that EHW inhibits the metabolism of ethanol to acetaldehyde by suppressing alcohol dehydrogenase. EHW also promotes the metabolism of acetaldehyde to acetic acid by activating aldehyde dehydrogenase, which plays to reduce aldehyde toxicity and intracellular reactive oxygen species in HepG2 cells. These functions were correlated with the concentration of molecular hydrogen in EHW, and were abolished by degassing treatment, suggesting that molecular hydrogen may contribute as a functional factor in the suppression of ethanol-induced hepatocellular damage. Furthermore, hydrogen water with high dissolved hydrogen molecule showed the same hepatocellular protective effect against ethanol as the EHW. These results suggest that EHW may be useful in the prevention of alcoholic liver disease.
登录
查看更多内容
影响因子：
3.7
作者：
Hamasaki T;Harada G;Nakamichi N;Kabayama S;Teruya K;Fugetsu B;Gong W;Sakata I;Shirahata S
通讯作者：
Shirahata S
DOI：
10.1080/23328940.2020.1742056
发表时间：
2020-04-17
期刊：
Temperature (Austin, Tex.)
影响因子：
--
作者：
Ito H;Kabayma S;Goto K
通讯作者：
Goto K
DOI：
10.1016/j.omtm.2019.08.004
发表时间：
2019-12-13
影响因子：
4.7
作者：
Matsumura, Yuki;Stiles, Katie M.;Crystal, Ronald G.
通讯作者：
Crystal, Ronald G.
影响因子：
2.2
作者：
Zhou, Jun;Chen, Ye;Wang, Jian
通讯作者：
Wang, Jian
酒精性肝病：发病机制和新的治疗靶点。
影响因子：
29.4
作者：
Gao B;Bataller R
通讯作者：
Bataller R