Reproductive injury in male BALB/c mice infected with Neospora caninum.
Reproductive injury in male BALB/c mice infected with Neospora caninum.
复制标题
感染犬新孢子虫的雄性 BALB/c 小鼠的生殖损伤
DOI：
10.1186/s13071-021-04664-y
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发表时间：
2021-03-16
影响因子：
3.2
通讯作者：
Jia LJ
中科院分区：
文献类型：
--
作者：
Li H;Yang BY;Liu MM;Zhao SW;Xie SZ;Wang H;Zhang S;Xuan XN;Jia LJ
背景
Background
Neospora caninum is one of the main causes of abortion in pregnant animals. However, N. caninum-induced reproductive injury in male mice is still unclear.
Methods
Male BALB/c mice were infected with a bovine isolate of N. caninum, and the organ coefficients of the testis and epididymis were measured. Lesions in the testis and epididymis were observed by light microscopy and transmission electron microscopy. Expression of the spermatogenic cell apoptosis-related proteins p53 and caspase-3 was detected by western blot. The expression of spermatogenesis-related genes in the testis was detected by reverse transcription-PCR. Sperm morphology and motility were observed. The levels of nitric oxide (NO) and antisperm antibody (AsAb) in the testicular homogenates and hormones in the serum were detected by enzyme-linked immunosorbent assay. The reproductive capacity of the male mice was detected using a reproduction test.
Results
The organ coefficients of the testis and epididymis of the experimental group were significantly downregulated. Light microscopy examination revealed that the spermatogenic cells of the testis were arranged in a disordered manner, and the number was reduced. The number of sperm in the epididymal lumen was significantly reduced, and the cytoplasm exhibited vacuolation and degeneration. Ultrastructural studies revealed that the cells of the testis and epididymis tissues showed varying degrees of disease. The level of p53 and caspase-3 expression in the testis was significantly upregulated. The expression of the testicular spermatogenesis-related genes Herc4, Ipo11 and Mrto4 were strongly downregulated. Observation of sperm by microscopic examination revealed significantly reduced sperm density and sperm motility, and the number of sperm deformities was significantly increased. The level of NO and AsAb was significantly increased. The levels of luteinizing hormone, follicle-stimulating hormone and gonadotropin-releasing hormone were significantly upregulated, whereas the levels of testosterone, thyrotropin-releasing hormone, thyroxine and thyroid-stimulating hormone were significantly downregulated. After challenge, the infected male mice and healthy female mice were caged together: the subsequent fetal death rate was increased, and the conception rate, litter size, number of live births and the birth weight were significantly reduced.
Conclusions
Infection of male BALB/c mice with the bovine isolate of N. caninum induced varying degrees of injury to the testis, epididymis and sperm of the mice, destroyed spermatogenesis and affected the reproductive capacity.
Graphical Abstract
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Genistein 通过青春期前暴露后激活 Nrf2/HO1 来减轻邻苯二甲酸二(2乙基己基)酯引起的睾丸损伤。
影响因子：
5.4
作者：
Zhang L;Li H;Gao M;Zhang T;Wu Z;Wang Z;Chong T
通讯作者：
Chong T
DOI：
10.1007/978-1-4939-7902-8_13
发表时间：
2018-01-01
期刊：
THYROID HORMONE NUCLEAR RECEPTOR: METHODS AND PROTOCOLS
影响因子：
--
作者：
Wondisford, F. E.
通讯作者：
Wondisford, F. E.
影响因子：
5.3
作者：
Yamada, D;Yoshida, M;Murakami, Y
通讯作者：
Murakami, Y
影响因子：
3.2
作者：
Villagra-Blanco R;Angelova L;Conze T;Schares G;Bärwald A;Taubert A;Hermosilla C;Wehrend A
通讯作者：
Wehrend A
DOI：
10.1051/parasite/2018019
发表时间：
2018
期刊：
Parasite (Paris, France)
影响因子：
--
作者：
Wang S;Li L;Lu Y;Zhang H;Xie Q;Zhang Z
通讯作者：
Zhang Z