Dynamic Changes in Lung Microbiota of Broilers in Response to Aging and Ammonia Stress.
Dynamic Changes in Lung Microbiota of Broilers in Response to Aging and Ammonia Stress.
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肉鸡肺部微生物群响应衰老和氨胁迫的动态变化
DOI：
10.3389/fmicb.2021.696913
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发表时间：
2021
影响因子：
5.2
通讯作者：
Sun Q
中科院分区：
文献类型：
--
作者：
Chen J;Jin A;Huang L;Zhao Y;Li Y;Zhang H;Yang X;Sun Q
综合微生物分析表明，肺部微生物区系种类繁多，虽然已经确定了不同日龄小鼠和蛋鸡肺微生物区系的动态分布，但这种分布在不同日龄肉鸡中的分布尚不清楚。在这里，我们对3(3天)、7(7天)、14(14天)、21(21天)和35(35天)龄肉鸡的肺灌洗液进行了16S rRNA基因测序，以评估它们肺微生物区系的变化。通过对肉鸡肺微生物区系组成和功能的研究，发现它们的成熟度随日龄的增加而显著增加。具体地说，7~14d和21~35d的微生物区系组成相似。肉鸡肺中需氧细菌的相对丰度随着肉鸡的发育逐渐增加，而潜在致病菌的相对丰度在3d时达到最高水平。微生物区系中预测功能的相对丰度在3、7和14d时非常相似，而在21d时微生物区系中糖的生物合成和代谢途径丰富。这些发现表明，这些代谢途径在形成这些年龄阶段的肉鸡微生物区系方面发挥了关键作用。此外，短期外氨刺激可显著增加肺部炎症，但对肺微生物区系无明显影响。综上所述，这些数据揭示了肉鸡肺部微生物区系随年龄的变化动态，以及这些微生物群落对短期氨胁迫的稳定性(微生物组成的显著变化)。这些发现为肉鸡肺部微生物区系的发展提供了新的见解，并为后续评估规模化养殖肉鸡疾病预防的研究提供了参考。
Comprehensive microbial analysis has revealed that the lung harbors a complex variety of microbiota, and although the dynamic distribution of the lung microbiota in mice and laying hens of different ages is well established, this distribution has not been clarified in broilers of different ages. Here, we performed 16S rRNA gene sequencing of lung lavage fluid from broilers at 3 (3D), 7 (7D), 14 (14D), 21 (21D), and 35 (35D) days of age to evaluate changes in the composition of their lung microbiota. Upon examination of the composition and function of the broiler lung microbiota, we found that their maturation increased significantly with age. Specifically, the microbiota composition was similar between 7 and 14D and between 21 and 35D. The relative abundance of aerobic bacteria in the broiler lungs gradually increased as the broilers developed, whereas the relative abundance of potentially pathogenic bacteria reached its highest level at 3D. The relative abundance of predicted functions in microbiota was very similar among 3, 7, and 14D, whereas the Glycan Biosynthesis and Metabolism pathway in microbiota was enriched at 21D. These findings suggest that these metabolic pathways play critical roles in shaping broiler microbiota at these age stages. In addition, short-term external ammonia stimulation significantly increased lung inflammation but did not significantly affect the lung microbiota. Taken together, these data reveal the dynamics of age-related changes in the microbiota of broiler lungs and the stability (the significant variation in the microbial composition) of these microbial communities in response to short-term ammonia stress. These findings provide new insights into the development of broiler lung microbiota and serve as a reference for subsequent studies to evaluate disease prevention in broilers subjected to large-scale breeding.
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DOI：
10.1126/science.1219328
发表时间：
2012-04-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Olszak T;An D;Zeissig S;Vera MP;Richter J;Franke A;Glickman JN;Siebert R;Baron RM;Kasper DL;Blumberg RS
通讯作者：
Blumberg RS
影响因子：
4.4
作者：
Liu QX;Zhou Y;Li XM;Ma DD;Xing S;Feng JH;Zhang MH
通讯作者：
Zhang MH
DOI：
10.1590/s1517-83822013000400002
发表时间：
2013-12
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Gomes ES;Schuch V;de Macedo Lemos EG
通讯作者：
de Macedo Lemos EG
DOI：
10.1152/ajplung.00061.2014
发表时间：
2015-07-01
影响因子：
4.9
作者：
Poroyko, Valeriy;Meng, Fanyong;Birukov, Konstantin G.
通讯作者：
Birukov, Konstantin G.
DOI：
10.1177/1559325818807790
发表时间：
2018-10
期刊：
Dose-response : a publication of International Hormesis Society
影响因子：
--
作者：
Tualeka AR;Faradisha J;Maharja R
通讯作者：
Maharja R