Two-photon NADH imaging exposes boundaries of oxygen diffusion in cortical vascular supply regions.
Two-photon NADH imaging exposes boundaries of oxygen diffusion in cortical vascular supply regions.
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DOI：
10.1038/jcbfm.2010.158
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发表时间：
2011-01
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影响因子：
--
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中科院分区：
文献类型：
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氧运输对大脑维持可变代谢需求的能力施加了可能的限制，但尚未直接观察到大脑皮层中的氧扩散。我们发现，内源性烟酰胺腺嘌呤二核苷酸（NADH）和皮质微循环的并发双光子荧光成像暴露在小鼠皮质组织氧扩散的明确边界。NADH荧光在p50为3.4±0.6 mm Hg的狭窄极低pO2范围内快速增加，从而建立了显著代谢限制性缺氧的近二元报告基因。短暂的皮质组织边界的NADH荧光表现出显着划定的几何图案，它定义了从皮质微循环的组织氧扩散的限制，并承担一个惊人的相似之处，理想的克罗组织圆柱体。微血管的可视化及其对氧气输送的区域贡献确立了穿透性小动脉作为除了毛细血管网络之外的主要氧气来源，并证实了具有陡峭的微区域氧气梯度的皮质氧场的存在。因此，双光子NADH成像可以应用于暴露血管供应区域，并以微米空间分辨率定位功能相关的微区域皮质缺氧。
Oxygen transport imposes a possible constraint on the brain's ability to sustain variable metabolic demands, but oxygen diffusion in the cerebral cortex has not yet been observed directly. We show that concurrent two-photon fluorescence imaging of endogenous nicotinamide adenine dinucleotide (NADH) and the cortical microcirculation exposes well-defined boundaries of tissue oxygen diffusion in the mouse cortex. The NADH fluorescence increases rapidly over a narrow, very low pO2 range with a p50 of 3.4±0.6 mm Hg, thereby establishing a nearly binary reporter of significant, metabolically limiting hypoxia. The transient cortical tissue boundaries of NADH fluorescence exhibit remarkably delineated geometrical patterns, which define the limits of tissue oxygen diffusion from the cortical microcirculation and bear a striking resemblance to the ideal Krogh tissue cylinder. The visualization of microvessels and their regional contribution to oxygen delivery establishes penetrating arterioles as major oxygen sources in addition to the capillary network and confirms the existence of cortical oxygen fields with steep microregional oxygen gradients. Thus, two-photon NADH imaging can be applied to expose vascular supply regions and to localize functionally relevant microregional cortical hypoxia with micrometer spatial resolution.
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影响因子：
5.5
作者：
Krogh, A
通讯作者：
Krogh, A
DOI：
10.1097/00004647-199701000-00009
发表时间：
1997-01-01
影响因子：
6.3
作者：
Buxton, RB;Frank, LR
通讯作者：
Frank, LR
DOI：
10.1073/pnas.0307458100
发表时间：
2004-01-13
影响因子：
11.1
作者：
Ido, Y;Chang, K;Williamson, JR
通讯作者：
Williamson, JR
影响因子：
56.9
作者：
PULSINELLI, WA;DUFFY, TE
通讯作者：
DUFFY, TE
影响因子：
4.4
作者：
Clay, G. Omar;Schaffer, Chris B.;Kleinfeld, David
通讯作者：
Kleinfeld, David