Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998-2002.

Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998-2002.
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DOI:
10.1007/s11250-006-4349-9
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发表时间:
2006-10
影响因子:
1.7
通讯作者:
Alfieri AF
Alfieri AF
中科院分区:
农林科学3区
文献类型:
--
作者:
Alfieri AA;Parazzi ME;Takiuchi E;Médici KC;Alfieri AF

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采用聚丙烯酰胺凝胶电泳技术测定了 1998 年至 2002 年犊牛中 A 组牛轮状病毒(gpA BRV)的频率,检测了 2177 份粪便样本,其中 1898 份为腹泻样本,279 份为正常稠度样本(对照组),这些样本是从无症状犊牛中收集的,用于比较。这些动物来自巴西七个州(巴拉那州、圣保罗州、米纳斯吉拉斯州、南马托格罗索州、马托格罗索州、戈亚斯州和朗多尼亚州)和四个巴西地理区域(南部、东南部、中西部和北部)158 个县的肉牛和奶牛群(n = 321)。在腹泻犊牛采集的样本中,19.4% (369/1898;p = 0.0001) 检测到了 GpA BRV,而在正常稠度的粪便中,仅 2.2% (6/279;p = 0.0001) 检测到了 GpA BRV。从肉牛群和奶牛群中采集的阳性样本比例分别为 22.8% (205/899; p = 0.0001) 和 16.4% (169/999; p = 0.0005)。与年龄相关,30 天以下的犊牛感染率较高,肉牛群和奶牛群的腹泻粪便样本中分别有 33.0%(189/573;p = 0.0001)和 20.2%(138/683;p = 0.0001)gpA BRV 呈阳性。这些结果表明,将 gpA BRV 纳入巴西牛群新生犊牛腹泻管理中可能具有重要意义。
The frequency of group A bovine rotavirus (gpA BRV) in calves from 1998 to 2002 was determined by the polyacrylamide gel electrophoresis technique in 2177 faecal samples, of which 1898 samples were diarrhoeic and 279 were of normal consistency (control group) that were collected from asymptomatic calves for comparative purposes. The animals were from beef and dairy cattle herds (n = 321) from 158 counties in seven States (Paraná, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso, Goiás and Rondônia) and four Brazilian geographical regions (south, south-east, centre-west, and north). GpA BRV was detected in 19.4% (369/1898; p = 0.0001) of the samples collected in calves with diarrhoea and in only 2.2% (6/279; p = 0.0001) of the faeces with normal consistency. The proportion of positive samples collected from beef and dairy cattle herds was 22.8% (205/899; p = 0.0001) and 16.4% (169/999; p = 0.0005), respectively. In relation to age, a higher prevalence of infections was found in calves up to 30 days old, where 33.0% (189/573; p = 0.0001) and 20.2% (138/683; p = 0.0001) of the diarrhoeic faecal samples from beef and dairy cattle herds, respectively, were positive for gpA BRV. These results show the possible importance of inclusion of gpA BRV in the management of neonatal calf diarrhoea in Brazilian cattle herds.
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