Coupled growth and division of model protocell membranes.
Coupled growth and division of model protocell membranes.
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DOI：
10.1021/ja900919c
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发表时间：
2009-04-22
影响因子：
15
通讯作者：
Szostak JW
中科院分区：
文献类型：
--
作者：
Zhu TF;Szostak JW
细胞生命的合成形式的产生需要解决诸如循环生长和分裂等生物过程如何从纯粹的物理和化学系统中产生的问题。当脂肪酸胶束喂养时，小的单层脂肪酸囊泡可以生长，并可以通过挤压强迫分裂，但这种人工分裂过程导致每个分裂周期中原始细胞内容物的显著损失。在这里，我们描述了一个简单而有效的模型原细胞膜生长和分裂的途径。在溶质通过膜渗透缓慢的溶液中，以脂肪酸胶束为原料的大型多层脂肪酸囊泡的生长，导致最初的球形囊泡转变为长丝状囊泡，这一过程是由表面积和体积增长之间的短暂不平衡驱动的。适度的剪切力足以使线状囊泡分裂成多个子囊泡而不会丢失内部内容物。在温和剪切的环境中，原始细胞的生长和分裂是耦合的过程。我们表明，模型原始细胞可以进行多个繁殖周期。被封装的RNA分子，代表一个原始基因组，分布到子囊泡。我们的观察使我们更接近于实验室合成一个完整的原始细胞，由一个自我复制的基因组和一个自我复制的膜室组成。此外，这一途径的稳健性和简单性表明，类似的过程可能在地球早期的益生元条件下发生过。
The generation of synthetic forms of cellular life requires solutions to the problem of how biological processes such as cyclic growth and division could emerge from purely physical and chemical systems. Small unilamellar fatty acid vesicles grow when fed with fatty acid micelles and can be forced to divide by extrusion, but this artificial division process results in significant loss of protocell contents during each division cycle. Here we describe a simple and efficient pathway for model protocell membrane growth and division. The growth of large multilamellar fatty acid vesicles fed with fatty acid micelles, in a solution where solute permeation across the membranes is slow, results in the transformation of initially spherical vesicles into long thread-like vesicles, a process driven by the transient imbalance between surface area and volume growth. Modest shear forces are then sufficient to cause the thread-like vesicles to divide into multiple daughter vesicles without loss of internal contents. In an environment of gentle shear, protocell growth and division are thus coupled processes. We show that model protocells can proceed through multiple cycles of reproduction. Encapsulated RNA molecules, representing a primitive genome, are distributed to the daughter vesicles. Our observations bring us closer to the laboratory synthesis of a complete protocell consisting of a self-replicating genome and a self-replicating membrane compartment. In addition, the robustness and simplicity of this pathway suggests that similar processes might have occurred under the prebiotic conditions of the early Earth.
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影响因子：
3.3
作者：
Berclaz, N;Müller, M;Luisi, PL
通讯作者：
Luisi, PL
影响因子：
56.9
作者：
Johnston, WK;Unrau, PJ;Bartel, DP
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DOI：
10.1073/pnas.172183699
发表时间：
2002-09-03
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
8.6
作者：
BARZIV, R;MOSES, E
通讯作者：
MOSES, E
DOI：
10.1073/pnas.0805086105
发表时间：
2008-09-09
影响因子：
11.1
作者：
Mansy, Sheref S.;Szostak, Jack W.
通讯作者：
Szostak, Jack W.