Microvascular density and vascular endothelial growth factor and osteopontin expression during the implantation window in a controlled ovarian hyperstimulation rat model.
Microvascular density and vascular endothelial growth factor and osteopontin expression during the implantation window in a controlled ovarian hyperstimulation rat model.
复制标题
DOI：
10.3892/etm.2015.2181
复制
发表时间：
2015-03
影响因子：
2.7
通讯作者：
Jin Z
中科院分区：
文献类型：
--
作者：
Gong X;Tong Q;Chen Z;Zhang Y;Xu C;Jin Z
血管内皮生长因子（VEGF）和骨桥蛋白（OPN）在子宫内膜容受性中促进血管生成和血管重构。子宫内膜微血管密度（MVD）的测定是间接评估血管生成和血管生成水平的最常用方法；然而，VEGF、OPN和MVD之间的关系尚不清楚。控制卵巢过度刺激（COH）与促性腺激素释放激素激动剂长期方案可能会损害子宫内膜容受性，中医（TCM）可能发挥治疗作用，以减轻这种损害。本研究旨在探讨COH对胚胎着床生物学和妊娠结局的影响，并探讨中药紫胆饮（ZDY）的潜在治疗作用。雌性sd大鼠分为对照组、COH组、ZDY组和COH + ZDY组。在妊娠第3、4、5天（分别为D3、D4、D5），采用分化簇34免疫组化检测子宫内膜MVD，采用western blotting和逆转录-定量聚合酶链反应（RT-qPCR）检测VEGF、OPN蛋白及mRNA表达。D10观察平均着床部位数。在怀孕和生育新生大鼠后，记录每组的活产数量。COH被证明对着床和妊娠结局有不良影响。与对照组、ZDY组和COH+ZDY组相比，COH组的MVD明显升高。VEGF和OPN的蛋白及RT-qPCR分析结果显示了相同的趋势。相反，ZDY逆转了子宫内膜MVD、VEGF和OPN的变化，并被认为可以改善子宫容受性和妊娠结局。对照组、ZDY组和COH + ZDY组间无显著差异。综上所述，由于MVD与VEGF和OPN表达的结果是一致的，MVD可以作为鉴定大鼠接受期的替代方法。
Vascular endothelial growth factor (VEGF) and osteopontin (OPN) are suggested to facilitate angiogenesis and vascular remodeling in endometrial receptivity. Determination of the endometrial microvascular density (MVD) is the commonest method used to indirectly assess the levels of vasculogenesis and angiogenesis; however, the associations among VEGF, OPN and MVD remain unclear. Controlled ovarian hyperstimulation (COH) with the gonadotrophin-releasing hormone agonist-long protocol may impair endometrial receptivity, and Traditional Chinese Medicine (TCM) may exert therapeutic effects to relieve this impairment. The aim of the present study was to investigate the effects of COH on implantation biology and pregnancy outcome, and to explore the potential therapeutic role of the TCM Zi Dan Yin (ZDY). Female Sprague Dawley rats were divided into four groups: Control, COH, ZDY and COH + ZDY. On days 3, 4 and 5 of pregnancy (D3, D4 and D5, respectively), endometrial MVD was measured with cluster of differentiation 34 immunohistochemical detection, and VEGF and OPN protein and mRNA expression was detected through western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. On D10, the average number of implantation sites was observed. Subsequent to conceiving and bearing newborn rats, the number of live births from each group was recorded. COH was shown to have adverse effects on implantation and pregnancy outcome. The MVD was found to be significantly increased in the COH group compared with that in the control, ZDY and COH+ZDY groups. The results of the protein and RT-qPCR analysis of VEGF and OPN revealed the same trend. Conversely, ZDY reversed the changes in endometrial MVD, VEGF and OPN, and was indicated to improve uterine receptivity and pregnancy outcome. No significant difference was observed among the control, ZDY and COH + ZDY groups. In conclusion, since the results for MVD and VEGF and OPN expression were consistent, MVD could be used as an alternative approach to identify the period of receptivity in rats.
登录
查看更多内容
影响因子：
3.7
作者：
Chaen T;Konno T;Egashira M;Bai R;Nomura N;Nomura S;Hirota Y;Sakurai T;Imakawa K
通讯作者：
Imakawa K
影响因子：
3.3
作者：
BROWN, LF;BERSE, B;SENGER, DR
通讯作者：
SENGER, DR
影响因子：
6.1
作者：
Ertzeid, G;Storeng, R
通讯作者：
Storeng, R
影响因子：
4
作者：
Borthwick, JM;Charnock-Jones, DS;Smith, SK
通讯作者：
Smith, SK
DOI：
10.1152/ajplung.1998.274.5.l678
发表时间：
1998-05-01
影响因子：
4.9
作者：
Hippenstiel, S;Krüll, M;Suttorp, N
通讯作者：
Suttorp, N