Printing support hydrogels for creating vascular-like structures in stacked cell sheets

Printing support hydrogels for creating vascular-like structures in stacked cell sheets
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打印支撑水凝胶,用于在堆叠的细胞片中创建血管样结构

DOI:
10.1007/s10015-020-00605-7
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发表时间:
2020
影响因子:
0.9
通讯作者:
Umezu Shinjiro
Umezu Shinjiro
中科院分区:
--
文献类型:
--
作者:
Tanaka Ryu-ichiro;Sakaguchi Katsuhisa;Umezu Shinjiro

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血管结构对于厚的人工三维(3D)组织的存活是必不可少的。然而,很难产生具有直径几百微米的血管结构的高细胞密度人造组织。生物打印技术可以制造出直径几百微米的人造3D组织,但生物打印的人造3D组织的细胞密度很低。另一方面,细胞片层技术可以通过堆叠来创建高细胞密度的人工3D组织,但不可能在任何地方设置小血管结构。在这项研究中,我们成功地展示了具有血管样结构的高细胞密度人工3D组织,通过堆叠细胞片结合生物打印技术。
Vascular structures are essential for the survival of thick artificial three-dimensional (3D) tissues. However, it is difficult to create high-cell-density artificial tissue with vascular structures of a few hundred micrometers in diameter. Bioprinting technology can create artificial 3D tissues with vascular structures of a few hundred micrometers in diameter, but the cell density of bio-printed artificial 3D tissues is low. On the other hand, cell sheet technology can create high-cell-density artificial 3D tissues by stacking, but it is not possible to set small vascular structures at any place. In this study, we successfully demonstrated high-cell-density artificial 3D tissues with vascular-like structures by stacking cell sheets combined with bioprinting technology.
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