Signal-to-noise and radiation exposure considerations in conventional and diffraction x-ray microscopy.

Signal-to-noise and radiation exposure considerations in conventional and diffraction x-ray microscopy.
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DOI:
10.1364/oe.17.013541
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发表时间:
2009-08-03
期刊:
影响因子:
3.8
通讯作者:
Jacobsen C
Jacobsen C
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Huang X;Miao H;Steinbrener J;Nelson J;Shapiro D;Stewart A;Turner J;Jacobsen C

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使用基于多个模拟图像之间的相关性的信噪比估计,我们比较了两种软X射线成像系统的剂量效率:在透射X射线显微镜(TXM)中使用波带片光学器件的非相干明场成像,以及从远场相干衍射图案重建图像的X射线衍射显微镜(XDM)。在XDM中,必须计算相位弱衍射信号;在TXM中,由于光学器件的有限数值孔径和效率,人们遭受信号损失。在模拟对象代表孤立的细胞,如酵母,我们发现,XDM有潜力提供同等分辨率的图像使用更少的光子。这对于研究对辐射敏感的生物和软物质标本来说是一个重要的优势。
Using a signal-to-noise ratio estimation based on correlations between multiple simulated images, we compare the dose efficiency of two soft x-ray imaging systems: incoherent brightfield imaging using zone plate optics in a transmission x-ray microscope (TXM), and x-ray diffraction microscopy (XDM) where an image is reconstructed from the far-field coherent diffraction pattern. In XDM one must computationally phase weak diffraction signals; in TXM one suffers signal losses due to the finite numerical aperture and efficiency of the optics. In simulations with objects representing isolated cells such as yeast, we find that XDM has the potential for delivering equivalent resolution images using fewer photons. This can be an important advantage for studying radiation-sensitive biological and soft matter specimens.
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