Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction.
Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction.
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腐败希瓦氏菌 W3-18-1 的 UndA 和 MtrC 在铁还原中的作用
DOI：
10.1186/1471-2180-13-267
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发表时间：
2013-11-25
期刊：
影响因子：
4.2
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Yang Y;Chen J;Qiu D;Zhou J
一些以金属还原能力而闻名的希瓦氏菌物种的基因组测序工作的完成，为比较研究提供了基础。早先在白氏杆菌MR-1中的研究表明，一个簇(mtrBAC-OMCA-mtrFED)中的一些基因参与了铁还原。为了探索铁还原途径的新特征，我们对腐败希瓦氏杆菌W3-18-1进行了实验分析，因为它的基因簇与MR-1的基因簇有很大的不同，因为基因簇只编码4个ORF。在基因簇中，有两个基因(mtrC和undA)编码C型细胞色素。ΔmtrC缺失突变体在还原金属Fe_2O_3、α-FeO(OH)、β-FeO(OH)、柠檬酸铁、Mn(IV)和Co(III)方面存在显著缺陷，但不包括有机化合物。相反，在ΔundA缺失突变体中没有观察到金属还原缺陷。尽管如此，在没有mtrC的情况下，undA缺失导致铁还原逐渐变慢，并且在用铁条件下的适应性丧失，这表明undA在铁还原中发挥了作用。这些结果为腐败希瓦氏菌W3-18-1的undA和mtrC参与铁还原提供了生理生化证据。
The completion of genome sequencing in a number of Shewanella species, which are most renowned for their metal reduction capacity, offers a basis for comparative studies. Previous work in Shewanella oneidensis MR-1 has indicated that some genes within a cluster (mtrBAC-omcA-mtrFED) were involved in iron reduction. To explore new features of iron reduction pathways, we experimentally analyzed Shewanella putrefaciens W3-18-1 since its gene cluster is considerably different from that of MR-1 in that the gene cluster encodes only four ORFs. Among the gene cluster, two genes (mtrC and undA) were shown to encode c-type cytochromes. The ΔmtrC deletion mutant revealed significant deficiencies in reducing metals of Fe2O3, α-FeO(OH), β-FeO(OH), ferric citrate, Mn(IV) and Co(III), but not organic compounds. In contrast, no deficiency of metal reduction was observed in the ΔundA deletion mutant. Nonetheless, undA deletion resulted in progressively slower iron reduction in the absence of mtrC and fitness loss under the iron-using condition, which was indicative of a functional role of UndA in iron reduction. These results provide physiological and biochemical evidences that UndA and MtrC of Shewanella putrefaciens W3-18-1 are involved in iron reduction.
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影响因子：
5
作者：
Salas, Everett C.;Berelson, William M.;Hammond, Douglas E.;Kampf, Anthony R.;Nealson, Kenneth H.
通讯作者：
Nealson, Kenneth H.
影响因子：
4.4
作者：
Gao H;Wang X;Yang ZK;Palzkill T;Zhou J
通讯作者：
Zhou J
DOI：
10.1073/pnas.0902000106
发表时间：
2009-09-15
影响因子：
11.1
作者：
Konstantinidis, Konstantinos T.;Serres, Margrethe H.;Tiedje, James M.
通讯作者：
Tiedje, James M.
影响因子：
4.4
作者：
Yang Y;Harris DP;Luo F;Xiong W;Joachimiak M;Wu L;Dehal P;Jacobsen J;Yang Z;Palumbo AV;Arkin AP;Zhou J
通讯作者：
Zhou J
影响因子：
4.4
作者：
Lower, Brian H.;Yongsunthon, Ruchirej;Lower, Steven K.
通讯作者：
Lower, Steven K.