Dual transneuronal tracing in the rat entorhinal-hippocampal circuit by intracerebral injection of recombinant rabies virus vectors.
Dual transneuronal tracing in the rat entorhinal-hippocampal circuit by intracerebral injection of recombinant rabies virus vectors.
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DOI：
10.3389/neuro.05.001.2009
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发表时间：
2009
影响因子：
2.9
通讯作者：
Iijima T
中科院分区：
文献类型：
--
作者：
Ohara S;Inoue K;Yamada M;Yamawaki T;Koganezawa N;Tsutsui K;Witter MP;Iijima T
双跨神经元示踪是一种新的病毒示踪方法，它采用两种重组病毒，每种病毒表达不同的报告蛋白。外周注射重组伪狂犬病病毒已被用作一种强有力的方法来定义神经元，协调输出到运动和自主神经系统的各种外周靶点。在这里，我们评估了狂犬病病毒（RV）载体的重组体的双重跨神经元示踪在中枢神经系统的可行性。首先，我们研究了两种不同的RV载体是否可以在体外双标记细胞，并表明可以通过在短时间间隔内用两种RV载体感染靶细胞来实现有效的双标记。双transneuronal跟踪的潜力，然后在体内检查内嗅-海马电路，使用链的预测从CA 3锥体细胞CA 1锥体细胞，随后内嗅皮质。将两种RV载体分别注射到左右内嗅皮层后6天，在双侧CA 3区观察到双标记神经元。部分双标神经元呈高尔基体样标记。双transneuronal跟踪潜在地提供了一个强大的和敏感的方法来研究的问题，如在中枢神经系统中的电路内和之间的收敛和发散的量。使用这种敏感的技术，我们建立了在CA 3的单个神经元连接到内嗅皮层双边只有一个突触中继。
Dual transneuronal tracing is a novel viral tracing methodology which employs two recombinant viruses, each expressing a different reporter protein. Peripheral injection of recombinant pseudorabies viruses has been used as a powerful method to define neurons that coordinate outputs to various peripheral targets of motor and autonomic systems. Here, we assessed the feasibility of recombinants of rabies virus (RV) vector for dual transneuronal tracing in the central nervous system. First, we examined whether two different RV-vectors can double label cells in vitro, and showed that efficient double labeling can be realized by infecting targeted cells with the two RV-vectors within a short time interval. The potential of dual transneuronal tracing was then examined in vivo in the entorhinal-hippocampal circuit, using the chain of projections from CA3 pyramidal cells to CA1 pyramidal cells and subsequently to entorhinal cortex. Six days after the injection of two RV-vectors into the left and right entorhinal cortex respectively, double-labeled neurons were observed in CA3 bilaterally. Some double-labeled neurons showed a Golgi-like labeling. Dual transneuronal tracing potentially provides a powerful and sensitive method to study issues such as the amount of convergence and divergence within and between circuits in the central nervous system. Using this sensitive technique, we established that single neurons in CA3 are connected to the entorhinal cortex bilaterally with only one synaptic relay.
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影响因子：
46.9
作者：
Nagai, T;Ibata, K;Miyawaki, A
通讯作者：
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作者：
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