An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system.
An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system.
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DOI：
10.1038/srep39232
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发表时间：
2016-12-16
影响因子：
4.6
通讯作者：
Takahashi F
中科院分区：
文献类型：
--
作者：
Kasahara M;Suetsugu N;Urano Y;Yamamoto C;Ohmori M;Takada Y;Okuda S;Nishiyama T;Sakayama H;Kohchi T;Takahashi F
腺苷酸环化酶（AC）产生信号分子cAMP，在从原核生物到真核生物的各种生物中具有许多重要的细胞功能。在这里，我们报道了从多形地茅中鉴定和表征一个AC基因。该编码蛋白具有类似III类AC的c端AC催化结构域和降解环核苷酸的n端环核苷酸磷酸二酯酶（PDE）结构域，因此我们将该基因命名为MpCAPE （COMBINED AC with PDE）。重组蛋白的生化分析表明，MpCAPE具有AC和PDE活性。在MpCAPE启动子GUS系中，GUS活性在雄性性器官睾丸中被特异性检测到，这表明MpCAPE和cAMP信号可能参与了雄性生殖过程。CAPE同源物仅分布在以活动精子为雄性配子的陆生植物和蕨类植物中。CAPE是III类AC的一个亚类，可能在基生植物雄性器官和细胞发育中起重要作用。
Adenylyl cyclase (AC), which produces the signalling molecule cAMP, has numerous important cellular functions in diverse organisms from prokaryotes to eukaryotes. Here we report the identification and characterization of an AC gene from the liverwort Marchantia polymorpha. The encoded protein has both a C-terminal AC catalytic domain similar to those of class III ACs and an N-terminal cyclic nucleotide phosphodiesterase (PDE) domain that degrades cyclic nucleotides, thus we designated the gene MpCAPE (COMBINED AC with PDE). Biochemical analyses of recombinant proteins showed that MpCAPE has both AC and PDE activities. In MpCAPE-promoter-GUS lines, GUS activity was specifically detected in the male sexual organ, the antheridium, suggesting MpCAPE and thus cAMP signalling may be involved in the male reproductive process. CAPE orthologues are distributed only in basal land plants and charophytes that use motile sperm as the male gamete. CAPE is a subclass of class III AC and may be important in male organ and cell development in basal plants.
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DOI：
10.1186/1478-811x-8-15
发表时间：
2010-06-25
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Gehring C
通讯作者：
Gehring C
影响因子：
5.3
作者：
FIELD, J;NIKAWA, J;WIGLER, M
通讯作者：
WIGLER, M
影响因子：
16.6
作者：
Hori K;Maruyama F;Fujisawa T;Togashi T;Yamamoto N;Seo M;Sato S;Yamada T;Mori H;Tajima N;Moriyama T;Ikeuchi M;Watanabe M;Wada H;Kobayashi K;Saito M;Masuda T;Sasaki-Sekimoto Y;Mashiguchi K;Awai K;Shimojima M;Masuda S;Iwai M;Nobusawa T;Narise T;Kondo S;Saito H;Sato R;Murakawa M;Ihara Y;Oshima-Yamada Y;Ohtaka K;Satoh M;Sonobe K;Ishii M;Ohtani R;Kanamori-Sato M;Honoki R;Miyazaki D;Mochizuki H;Umetsu J;Higashi K;Shibata D;Kamiya Y;Sato N;Nakamura Y;Tabata S;Ida S;Kurokawa K;Ohta H
通讯作者：
Ohta H
影响因子：
7.4
作者：
ASSMANN, SM
通讯作者：
ASSMANN, SM
影响因子：
3.5
作者：
Al-Younis, Inas;Wong, Aloysius;Gehring, Chris
通讯作者：
Gehring, Chris