ABCG2-overexpressing H460/MX20 cell xenografts in athymic nude mice maintained original biochemical and cytological characteristics.
ABCG2-overexpressing H460/MX20 cell xenografts in athymic nude mice maintained original biochemical and cytological characteristics.
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无胸腺裸鼠中ABCG2过表达的H460/MX20细胞异种移植物保持了原始的生化和细胞学特征
DOI：
10.1038/srep40064
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发表时间：
2017-01-06
影响因子：
4.6
通讯作者：
Fu L
中科院分区：
文献类型：
--
作者：
Zhang W;Chen Z;Chen L;Wang F;Li F;Wang X;Fu L
H460/MX20细胞来源于大细胞肺癌H460细胞系，经米托蒽醌诱导后转化为ABCG 2高表达细胞，广泛应用于体外多药耐药（MDR）研究。为建立和推广H460/MX20细胞异种移植模型，我们研究了H460/MX20细胞在体内移植模型中的生物学特性和MDR表型是否得以保持。我们的结果表明，从H460/MX20细胞异种移植物中分离的xH 460/MX20细胞的细胞增殖、细胞周期和ABCG 2表达水平与体外培养的H460/MX20细胞相似。重要的是，xH 460/MX20细胞与H460/MX20细胞一样，对ABCG 2底物如米托蒽醌和拓扑替康表现出高水平的耐药性。此外，ABCG 2抑制剂拉帕替尼可有效逆转xH 460/MX20细胞对米托蒽醌和托泊替康的耐药性。综上所述，这些结果表明，H460/MX 20细胞在无胸腺裸鼠中的异种移植物仍然保留其原始细胞学特征和MDR表型。因此，H460/MX20细胞移植瘤模型可作为研究MDR逆转的体内模型。
H460/MX20 are derived from large cell lung cancer H460 cell line and then transformed into ABCG2-overexpressing cells by mitoxantrone’s induction, which are widely used in study of multidrug resistance (MDR) in vitro. To establish and spread the model of H460/MX20 cell xenografts, we investigated whether cell biological characteristics and the MDR phenotype were maintained in vivo model. Our results demonstrated that the cell proliferation, cell cycle, and ABCG2 expression level in xH460/MX20 cells isolated from H460/MX20 cell xenografts were similar to H460/MX20 cells in vitro. Importantly, xH460/MX20 cells exhibited high levels of resistance to ABCG2 substrates such as mitoxantrone and topotecan as H460/MX20 cells did. Furthermore, lapatinib, the inhibitor of ABCG2, potently reversed mitoxantrone- and topotecan-resistance of xH460/MX20 cells. Taken together, these results suggest that H460/MX20 cell xenografts in athymic nude mice still retain their original cytological characteristics and MDR phenotype. Thus, the H460/MX20 cell xenografts model could serve as a sound model in vivo for study on reversal MDR.
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影响因子：
--
作者：
Wang XK;To KK;Huang LY;Xu JH;Yang K;Wang F;Huang ZC;Ye S;Fu LW
通讯作者：
Fu LW
影响因子：
4.8
作者：
Evseenko, Denis A.;Murthi, Padma;Keelan, J. A.
通讯作者：
Keelan, J. A.
影响因子：
5.7
作者：
Henrich, Curtis J.;Robey, Robert W.;McMahon, James B.
通讯作者：
McMahon, James B.
DOI：
10.1615/jenvironpatholtoxicoloncol.2013006767
发表时间：
2012-01-01
影响因子：
2.4
作者：
Omran, Ola M.
通讯作者：
Omran, Ola M.
影响因子：
3.8
作者：
Evseenko, D. A.;Paxton, J. W.;Keelan, J. A.
通讯作者：
Keelan, J. A.