Dynamics of membrane potential variation and gene expression induced by Spodoptera littoralis, Myzus persicae, and Pseudomonas syringae in Arabidopsis.
Dynamics of membrane potential variation and gene expression induced by Spodoptera littoralis, Myzus persicae, and Pseudomonas syringae in Arabidopsis.
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DOI：
10.1371/journal.pone.0046673
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Maffei ME
中科院分区：
文献类型：
--
作者：
Bricchi I;Bertea CM;Occhipinti A;Paponov IA;Maffei ME
由各种植食动物和病原体诱导的生物胁迫引起植物的反应涉及不同的防御机制。然而，我们不知道是否不同的生物胁迫共享一个共同的反应，或者哪些信号通路参与了对不同生物胁迫的反应。我们研究了拟南芥对三种生物胁迫因子：Spodoptera littoralis，Myzus persicae和病原菌Pseudomonasjuveningae的共同和特异性反应。我们使用电生理学来确定质膜电位（Vm），我们进行了基因微阵列转录组分析拟南芥无论是草食动物或细菌感染。昆虫攻击可引起Vm去极化，但对S. littoralis（30 min-2 h）比M.桃蚜（4-6 h）。M. persicae差异调节的基因几乎是S. littoralis与相反的规则。M.桃蚜对类黄酮、脂肪酸、激素、药物转运和几丁质代谢等基因的调控。S. littoralis调节对热、转录和离子转运的反应。最晚的Vm去极化（16 h）被发现为P. dingae。病原体调节水杨酸，茉莉酸和微生物的反应。尽管有这种迟发反应，但由P. dingae差异调节的基因数量更接近于由S. littoralis比M.桃蚜。拟南芥质膜响应Vm去极化的时间取决于生物攻击的性质，这允许设置一个时间点比较全基因组分析。Vm去极化与基因表达之间存在明显的相关性。persicae比S.海滨鱼蚜虫和病原菌之间几乎完全相反的调节，前者抑制而后者激活拟南芥的防御反应。
Biotic stress induced by various herbivores and pathogens invokes plant responses involving different defense mechanisms. However, we do not know whether different biotic stresses share a common response or which signaling pathways are involved in responses to different biotic stresses. We investigated the common and specific responses of Arabidopsis thaliana to three biotic stress agents: Spodoptera littoralis, Myzus persicae, and the pathogen Pseudomonas syringae. We used electrophysiology to determine the plasma membrane potential (Vm) and we performed a gene microarray transcriptome analysis on Arabidopsis upon either herbivory or bacterial infection. Vm depolarization was induced by insect attack; however, the response was much more rapid to S. littoralis (30 min −2 h) than to M. persicae (4–6 h). M. persicae differentially regulated almost 10-fold more genes than by S. littoralis with an opposite regulation. M. persicae modulated genes involved in flavonoid, fatty acid, hormone, drug transport and chitin metabolism. S. littoralis regulated responses to heat, transcription and ion transport. The latest Vm depolarization (16 h) was found for P. syringae. The pathogen regulated responses to salicylate, jasmonate and to microorganisms. Despite this late response, the number of genes differentially regulated by P. syringae was closer to those regulated by S. littoralis than by M. persicae. Arabidopsis plasma membranes respond with a Vm depolarization at times depending on the nature of biotic attack which allow setting a time point for comparative genome-wide analysis. A clear relationship between Vm depolarization and gene expression was found. At Vm depolarization timing, M. persicae regulates a wider array of Arabidopsis genes with a clear and distinct regulation than S. littoralis. An almost completely opposite regulation was observed between the aphid and the pathogen, with the former suppressing and the latter activating Arabidopsis defense responses.
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影响因子：
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