Brassinosteroids Regulate OFP1, a DLT Interacting Protein, to Modulate Plant Architecture and Grain Morphology in Rice.
Brassinosteroids Regulate OFP1, a DLT Interacting Protein, to Modulate Plant Architecture and Grain Morphology in Rice.
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油菜素类固醇调节 OFP1（一种 DLT 相互作用蛋白）来调节水稻的植物结构和谷物形态
DOI：
10.3389/fpls.2017.01698
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发表时间：
2017
影响因子：
5.6
通讯作者：
Chu C
中科院分区：
文献类型：
--
作者：
Xiao Y;Liu D;Zhang G;Tong H;Chu C
水稻的重要农艺性状，包括株高、叶片角度和籽粒大小，都是由类甾醇（BR）调节的。然而，其基本机制仍未完全了解。我们先前表明，GSK2，BR信号传导的中心负调节因子，靶向DLT，GRAS家族蛋白，以调节BR反应。在这里，我们确定了Ovate家族蛋白1（OFP 1）作为DLT相互作用蛋白。OFP 1广泛表达，定位于细胞质和细胞核。OFP 1的过表达导致叶角增大，株高降低，粒形改变，很大程度上类似于DLT过表达植株。遗传分析表明，OFP 1对植物构型的调控依赖于DLT功能。此外，我们还发现OFP 1在BR处理后有很强的诱导作用，并且BR信号转导的关键转录因子OsBZR 1与OFP 1启动子之间存在物理联系。此外，我们发现，赤霉素的合成大大抑制OFP 1过表达植物，表明OFP 1参与抑制植物生长的高BR或BR信号升高。此外，我们发现OFP 1直接与GSK 2激酶相互作用，抑制激酶活性显着促进OFP 1蛋白在植物中的积累。综上所述，我们确定OFP 1作为BR反应的额外调节因子，并揭示了BR如何在转录和蛋白质水平上促进OFP 1调节水稻的植株结构和籽粒形态。
Brassinosteroids (BRs) regulate important agronomic traits in rice, including plant height, leaf angle, and grain size. However, the underlying mechanisms remain not fully understood. We previously showed that GSK2, the central negative regulator of BR signaling, targets DLT, the GRAS family protein, to regulate BR responses. Here, we identified Ovate Family Protein 1 (OFP1) as a DLT interacting protein. OFP1 was ubiquitously expressed and the protein was localized in both cytoplasm and nucleus. Overexpression of OFP1 led to enlarged leaf angles, reduced plant height, and altered grain shape, largely resembled DLT overexpression plants. Genetic analysis showed that the regulation of plant architecture by OFP1 depends on DLT function. In addition, we found OFP1 was greatly induced by BR treatment, and OsBZR1, the critical transcription factor of BR signaling, was physically associated with the OFP1 promoter. Moreover, we showed that gibberellin synthesis was greatly repressed in OFP1 overexpression plants, suggesting OFP1 participates in the inhibition of plant growth by high BR or elevated BR signaling. Furthermore, we revealed that OFP1 directly interacts with GSK2 kinase, and inhibition of the kinase activity significantly promotes OFP1 protein accumulation in plant. Taken together, we identified OFP1 as an additional regulator of BR responses and revealed how BRs promote OFP1 at both transcription and protein levels to modulate plant architecture and grain morphology in rice.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
DOI：
10.1073/pnas.0501181102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Hackbusch, J;Richter, K;Uhrig, JF
通讯作者：
Uhrig, JF
影响因子：
11.6
作者：
Tanabe, S;Ashikari, M;Iwasaki, Y
通讯作者：
Iwasaki, Y
影响因子：
5.2
作者：
Schmitz, Aaron J.;Begcy, Kevin;Walia, Harkamal
通讯作者：
Walia, Harkamal
影响因子：
21.3
作者：
Gudesblat, Gustavo E.;Schneider-Pizon, Joanna;Russinova, Eugenia
通讯作者：
Russinova, Eugenia