Circadian rhythms persist without transcription in a eukaryote.
Circadian rhythms persist without transcription in a eukaryote.
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DOI：
10.1038/nature09654
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发表时间：
2011-01-27
期刊：
影响因子：
64.8
通讯作者：
Millar, Andrew J.
中科院分区：
文献类型：
--
作者：
O'Neill, John S.;van Ooijen, Gerben;Dixon, Laura E.;Troein, Carl;Corellou, Florence;Bouget, Francois-Yves;Reddy, Akhilesh B.;Millar, Andrew J.
Circadian rhythms are ubiquitous in eukaryotes, and co-ordinate numerous aspects of behaviour, physiology and metabolism, from sleep/wake cycles in mammals to growth and photosynthesis in plants. This daily timekeeping is thought to be driven by transcriptional/translational feedback loops, whereby rhythmic expression of clock gene products regulates expression of associated genes in approximately 24-hour cycles. The specific transcriptional components differ between phylogenetic kingdoms. The unicellular pico-eukaryotic alga, Ostreococcus tauri, possesses a naturally minimised clock, which includes many features that are shared with higher eukaryotes (plants), such as a central negative feedback loop that involves the morning-expressed CCA1 and evening-expressed TOC1 genes. Given that recent observations in animals and plants have revealed prominent post-translational contributions to timekeeping, a reappraisal of the transcriptional contribution to oscillator function is overdue. Here we show that non-transcriptional mechanisms are sufficient to sustain circadian timekeeping in the eukaryotic lineage, though they normally function in conjunction with transcriptional components. We identify oxidation of peroxiredoxin proteins as a transcription-independent rhythmic biomarker, which is also rhythmic in mammals. Moreover we show that pharmacological modulators of the mammalian clockwork have the same effects on rhythms in Ostreococcus. Post-translational mechanisms, and at least one rhythmic marker, appear to be better conserved than transcriptional clock regulators. It is plausible that the oldest oscillator components are non-transcriptional in nature, as in cyanobacteria, and are conserved across kingdoms.
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DOI：
10.1111/j.1742-4658.2009.06985.x
发表时间：
2009-05
期刊：
The FEBS journal
影响因子：
--
作者：
Hall A;Karplus PA;Poole LB
通讯作者：
Poole LB
DOI：
10.1126/science.1152506
发表时间：
2008-05-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
O'Neill JS;Maywood ES;Chesham JE;Takahashi JS;Hastings MH
通讯作者：
Hastings MH
影响因子：
9.9
作者：
通讯作者：
--
影响因子：
7.2
作者：
Baier, M;Dietz, KJ
通讯作者：
Dietz, KJ
DOI：
10.1073/pnas.0811410106
发表时间：
2008-12-30
影响因子：
11.1
作者：
Hirota, Tsuyoshi;Lewis, Warren G.;Kay, Steve A.
通讯作者：
Kay, Steve A.