CircINSR Regulates Fetal Bovine Muscle and Fat Development.
CircINSR Regulates Fetal Bovine Muscle and Fat Development.
复制标题
CircINSR 调节胎牛肌肉和脂肪发育
DOI：
10.3389/fcell.2020.615638
复制
发表时间：
2020
影响因子：
5.5
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Shen X;Tang J;Ru W;Zhang X;Huang Y;Lei C;Cao H;Lan X;Chen H
家畜肌肉发育水平直接影响家畜生产效率，而肌内脂肪(IMF)含量是影响肉品品质的重要因素。然而，环状RNA(CircRNA)影响肌肉和IMF发育的分子机制在很大程度上仍不清楚。在本研究中，我们从胎牛骨骼肌中分离出成肌细胞和肌内前脂肪细胞。油红O和BODIPY染色鉴定前脂肪细胞中的脂滴，抗肌球蛋白重链(MyHC)免疫荧光鉴定分化为成肌细胞的肌管。用生物信息学、双荧光报告系统、RNA下拉和RNA结合蛋白免疫沉淀等方法研究了CircINSR与microRNA(MiR)-15/16家族之间的相互作用。用分子和生化方法证实了CircINSR在成肌细胞和肌内前脂肪细胞中的作用。我们发现分离的成肌细胞和前脂肪细胞能够正常分化。CircINSR被发现作为miR-15/16家族的海绵，其靶向是CCND1和Bcl-2。CircINSR过表达显著促进成肌细胞和前脂肪细胞的增殖，抑制细胞凋亡。此外，CircINSR通过减轻miR-15/16对靶基因FOXO1和EPT1的抑制而抑制前脂肪细胞的脂肪生成。综上所述，我们的研究表明，CircINSR对胚胎肌肉和IMF的发育具有调节作用。
The level of muscle development in livestock directly affects the production efficiency of livestock, and the contents of intramuscular fat (IMF) is an important factor that affects meat quality. However, the molecular mechanisms through which circular RNA (circRNA) affects muscle and IMF development remains largely unknown. In this study, we isolated myoblasts and intramuscular preadipocytes from fetal bovine skeletal muscle. Oil Red O and BODIPY staining were used to identify lipid droplets in preadipocytes, and anti-myosin heavy chain (MyHC) immunofluorescence was used to identify myotubes differentiated from myoblasts. Bioinformatics, a dual-fluorescence reporter system, RNA pull-down, and RNA-binding protein immunoprecipitation were used to determine the interactions between circINSR and the micro RNA (miR)-15/16 family. Molecular and biochemical assays were used to confirm the roles played by circINSR in myoblasts and intramuscular preadipocytes. We found that isolated myoblasts and preadipocytes were able to differentiate normally. CircINSR was found to serve as a sponge for the miR-15/16 family, which targets CCND1 and Bcl-2. CircINSR overexpression significantly promoted myoblast and preadipocyte proliferation and inhibited cell apoptosis. In addition, circINSR inhibited preadipocyte adipogenesis by alleviating the inhibition of miR-15/16 against the target genes FOXO1 and EPT1. Taken together, our study demonstrated that circINSR serves as a regulator of embryonic muscle and IMF development.
登录
查看更多内容
影响因子：
2.9
作者：
Hoashi S;Hinenoya T;Tanaka A;Ohsaki H;Sasazaki S;Taniguchi M;Oyama K;Mukai F;Mannen H
通讯作者：
Mannen H
影响因子：
3.9
作者：
Beier, Justus P.;Bitto, Franz F.;Kneser, Ulrich
通讯作者：
Kneser, Ulrich
影响因子：
4.6
作者：
Barrett, Steven P.;Salzman, Julia
通讯作者：
Salzman, Julia
影响因子：
64.8
作者：
Chendrimada, TP;Gregory, RI;Shiekhattar, R
通讯作者：
Shiekhattar, R
影响因子：
4.8
作者：
Armoni, Michal;Harel, Chava;Karnieli, Eddy
通讯作者：
Karnieli, Eddy