Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese black cattle.
Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese black cattle.
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DOI：
10.1186/1471-2156-9-84
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发表时间：
2008-12-11
期刊：
影响因子：
2.9
通讯作者：
Mannen H
中科院分区：
文献类型：
--
作者：
Hoashi S;Hinenoya T;Tanaka A;Ohsaki H;Sasazaki S;Taniguchi M;Oyama K;Mukai F;Mannen H
脂肪酸组成在牛肉风味和降低循环中的低密度脂蛋白胆固醇浓度方面已成为牛肉行业的重要特征。本研究探讨了脂肪细胞型脂肪酸结合蛋白(FABP_4)、肝X受体α(LXRα)、细胞色素b5(Cytb5)、长链酰辅酶A合成酶(ACSL)1、ACSL4和二酰甘油酰基转移酶2(DGAT2)6个基因的多态性与脂肪酸组成的关系。序列比较发现6个基因有14个单核苷酸多态。其中，FABP4中的I74V和V110M和LXRα中的G51E和V133I是非同义替换。以234头日本黑牛为材料，分析了各基因型与脂肪酸组分的相关性。FABP4I74V和LXRαV133I分别与肌内脂肪中棕榈油酸(C16：1，P=0.0086)和亚油酸(C18：2，P=0.0121)含量显著相关。本研究结果提示，FABP_4I74V和LXRαV133I两个基因多态性可能分别与日本黑牛肌内脂肪中棕榈油酸(FABP_4 I74V)和亚油酸(LXRαV133I)组成有关。尤其是FABP4 I74V对C16：1比例有极显著影响(P<0.01)，表明I/I纯合子的比例比V/V纯合子高0.5个百分点。
Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding protein (FABP4), liver X receptor α (LXRα), cytochrome b5 (Cyt b5), long-chain acyl-CoA synthetase (ACSL) 1, ACSL4 and diacylglycerol acyltransferase 2 (DGAT2) and fatty acid composition. Sequence comparisons revealed 14 single nucleotide polymorphisms in six genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXRα, were nonsynonymous substitutions. The associations between the genotypes and fatty acid compositions were analyzed by using 234 Japanese Black cattle. The genotypes of FABP4 I74V and LXRα V133I were significantly associated with palmitoleic acids (C16:1, P = 0.0086) and linoleic acid (C18:2, P = 0.0121) content in intramuscular fat, respectively. Our findings suggest that the two polymorphisms of FABP4 I74V and LXRα V133I might be genetic factors in part associated with palmitoleic acid (FABP4 I74V) and linoleic acid (LXRα V133I) composition in intramuscular fat of Japanese Black cattle, respectively. Especially, FABP4 I74V had highly significant effect (P < 0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.
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影响因子：
2.5
作者：
Hoashi, Shogo;Ashida, Nobuhisa;Mannen, Hideyuki
通讯作者：
Mannen, Hideyuki
影响因子：
2.4
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2.6
作者：
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影响因子：
2.7
作者：
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SATO, R
影响因子：
3.3
作者：
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通讯作者：
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