A novel virus genome discovered in an extreme environment suggests recombination between unrelated groups of RNA and DNA viruses.
A novel virus genome discovered in an extreme environment suggests recombination between unrelated groups of RNA and DNA viruses.
复制标题
DOI：
10.1186/1745-6150-7-13
复制
发表时间：
2012-06-11
期刊：
影响因子：
5.5
通讯作者：
Stedman KM
中科院分区：
文献类型：
--
作者：
Diemer GS;Stedman KM
众所周知，病毒是地球上最丰富的生物，但人们对它们的集体起源和进化史知之甚少。由于基因突变和嵌合体的比率非常高，目前还不可能解决已知的主要病毒组的深刻进化历史。随着大量新的序列数据可用于比较分析，元基因组学为建立更全面的病毒进化观点提供了潜在的手段。对来自炎热的酸性湖泊的病毒元基因组序列的生物信息学分析显示，一种环状的、推测为单链的DNA病毒编码一种主要的衣壳蛋白，与仅在单链RNA病毒中发现的相似。从湖泊沉积物中提取的天然DNA进行反向PCR扩增，证实了病毒全基因组的存在和环状构型。病毒基因组似乎是两个表面上不相关的病毒组之间的RNA-DNA重组事件的结果。对环境序列数据库中以相似构型排列的同源基因进行了检查，并从海洋环境中鉴定了三个相似的推定病毒基因组。这一结果表明存在一组广泛存在但以前未被检测到的病毒。这种独特的病毒基因组对病毒出现和进化的理论具有重要意义，因为病毒间RNA-DNA重组的机制尚未确定，而且几乎没有证据表明这种不同的病毒谱系之间存在遗传交换。本文由EK、MK(由PF提名)和AM审阅要获得完整的评论，请转到评论者评论部分。
Viruses are known to be the most abundant organisms on earth, yet little is known about their collective origin and evolutionary history. With exceptionally high rates of genetic mutation and mosaicism, it is not currently possible to resolve deep evolutionary histories of the known major virus groups. Metagenomics offers a potential means of establishing a more comprehensive view of viral evolution as vast amounts of new sequence data becomes available for comparative analysis. Bioinformatic analysis of viral metagenomic sequences derived from a hot, acidic lake revealed a circular, putatively single-stranded DNA virus encoding a major capsid protein similar to those found only in single-stranded RNA viruses. The presence and circular configuration of the complete virus genome was confirmed by inverse PCR amplification from native DNA extracted from lake sediment. The virus genome appears to be the result of a RNA-DNA recombination event between two ostensibly unrelated virus groups. Environmental sequence databases were examined for homologous genes arranged in similar configurations and three similar putative virus genomes from marine environments were identified. This result indicates the existence of a widespread but previously undetected group of viruses. This unique viral genome carries implications for theories of virus emergence and evolution, as no mechanism for interviral RNA-DNA recombination has yet been identified, and only scant evidence exists that genetic exchange occurs between such distinct virus lineages. This article was reviewed by EK, MK (nominated by PF) and AM. For the full reviews, please go to the Reviewers' comments section.
登录
查看更多内容
DOI：
10.1111/j.1749-6632.1980.tb27987.x
发表时间：
1980-01-01
影响因子：
5.2
作者：
Botstein, D
通讯作者：
Botstein, D
影响因子：
6.7
作者：
Chiba S;Kondo H;Tani A;Saisho D;Sakamoto W;Kanematsu S;Suzuki N
通讯作者：
Suzuki N
影响因子：
4.8
作者：
Heller-Dohmen M;Göpfert JC;Pfannstiel J;Spring O
通讯作者：
Spring O
影响因子：
3.9
作者：
Forterre, P
通讯作者：
Forterre, P
DOI：
10.1073/pnas.96.5.2192
发表时间：
1999-03-02
影响因子：
11.1
作者：
Hendrix, RW;Smith, MCM;Hatfull, GF
通讯作者：
Hatfull, GF