A reliable protocol for the manual segmentation of the human amygdala and its subregions using ultra-high resolution MRI.
A reliable protocol for the manual segmentation of the human amygdala and its subregions using ultra-high resolution MRI.
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DOI：
10.1016/j.neuroimage.2011.12.073
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发表时间：
2012-04-02
期刊：
影响因子：
5.7
通讯作者：
Dickerson, Bradford C.
中科院分区：
文献类型：
--
作者：
Entis, Jonathan J.;Doerga, Priya;Barrett, Lisa Feldman;Dickerson, Bradford C.
在过去的十年中，人体杏仁核体积的测量受到了越来越多的关注，但现有的方法面临着一些挑战。首先，由于杏仁核的无定形外观和解释其边界的困难，协议通常忽略杏仁核的吻侧和背侧区域的相当大的部分，分别包括基底外侧复合体（BL）和中央核（Ce）的部分。其次，由于常规采集图像的分辨率和缺乏标准协议，将杏仁复合体分割成单独的细分是具有挑战性的。技术的最新进展已经取得了超高分辨率的MR图像，在这项研究中，我们提供了一个详细的分割协议，手动跟踪整个杏仁核，结合了更大的一部分，吻侧和背侧部分的技术，详细说明，以最大限度地提高再现性。此外，我们提出了一个几何为基础的协议分割杏仁核成四个组成部分的利益（sROI），这在很大程度上对应于杏仁核亚核司：BL sROI，中央（CM）sROI，基底内侧（BM）sROI，和杏仁皮质（ACo）sROI。我们在10名成年人（5名年轻人和5名老年人）中对我们的方法进行了评价者内和评价者间可靠性研究。结果表明，这两种方案都可以以高度的可靠性实施（大多数评价者内和评价者间相关性>0.81）。该方案应有助于进一步研究杏仁核解剖结构，连接和功能的变化，伴随着正常的衰老和病理与神经精神疾病。
The measurement of the volume of the human amygdala in vivo has received increasing attention over the past decade, but existing methods face several challenges. First, due to the amorphous appearance of the amygdala and the difficulties in interpreting its boundaries, it is common for protocols to omit sizable sections of the rostral and dorsal regions of the amygdala comprising parts of the basolateral complex (BL) and central nucleus (Ce), respectively. Second, segmentation of the amgydaloid complex into separate subdivisions is challenging due to the resolution of routinely acquired images and the lack of standard protocols. Recent advances in technology have made ultra-high resolution MR images available, and in this study we provide a detailed segmentation protocol for manually tracing the whole amygdala that incorporates a greater portion of the rostral and dorsal sections with techniques illustrated in detail to maximize reproducibility. In addition, we propose a geometrically-based protocol for segmenting the amygdala into four component subregions of interest (sROI), which correspond largely to amygdala subnuclear divisions: the BL sROI, centromedial (CM) sROI, basomedial (BM) sROI, and the amygdaloid cortical (ACo) sROI. We performed an intra- and inter-rater reliability study of our methods in 10 adults (5 young adults and 5 older adults). The results indicate that both protocols can be implemented with a high degree of reliability (the majority of intra-rater and inter-rater correlations were >0.81). This protocol should aid further research into the alterations in amygdala anatomy, connectivity, and function that accompany normal aging and pathology associated with neuropsychiatric disorders.
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影响因子：
5.7
作者：
Fischl, B;Sereno, MI;Dale, AM
通讯作者：
Dale, AM
在大鼠和人类中持续的恐惧：恐惧与焦虑中的杏仁核的作用。
DOI：
10.1038/npp.2009.109
发表时间：
2010-01
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.7
作者：
Dale, AM;Fischl, B;Sereno, MI
通讯作者：
Sereno, MI
影响因子：
4.8
作者：
DICE, LR
通讯作者：
DICE, LR
影响因子：
5.7
作者：
Chupin, Marie;Mukuna-Bantumbakulu, A. Romain;Garnero, Line
通讯作者：
Garnero, Line