Characterization of a cultured dermal substitute composed of a spongy matrix of hyaluronic acid and collagen combined with fibroblasts
Characterization of a cultured dermal substitute composed of a spongy matrix of hyaluronic acid and collagen combined with fibroblasts
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由透明质酸和胶原蛋白与成纤维细胞组成的海绵状基质组成的培养真皮替代品的表征
DOI：
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发表时间：
2003
影响因子：
1.3
通讯作者：
Y. Kuroyanagi
中科院分区：
文献类型：
--
作者：
K. Kubo;Y. Kuroyanagi
作者通过在透明质酸(HA)和胶原(COL)双层海绵基质上培养成纤维细胞，研制出一种同种异体培养真皮替代物。Col海绵层对于成纤维细胞在双层海绵基质上的附着和增殖是必不可少的。透明质酸海绵层是维持伤口表面湿润环境所必需的。HA/COL的最佳重量比是通过考虑以下特性来确定的：处理的力学性能、解冻后的细胞活力、解冻后释放的血管内皮生长因子(VEGF)的效力、创面愈合的有效性和制造成本。本研究旨在研究CDS的物理性质、成纤维细胞在海绵基质上的生长行为，以及在新鲜和冷冻保存的CDS中成纤维细胞释放的血管内皮生长因子的定量分析。本研究的结果表明，单独由Col海绵基质组成的CDS具有最高的释放VEGF的效力。然而，考虑到制造成本，再加上血管内皮生长因子释放的效力，重量比为5/2的HA和Col双层海绵是非常有希望的商业应用。
Abstract The authors have developed an allogeneic cultured dermal substitute (CDS) through cultivation of fibroblasts on a two-layered spongy matrix of hyaluronic acid (HA) and atelocollagen (Col). The Col spongy layer is essential for attachment and proliferation of fibroblasts on the two-layered spongy matrix. The HA spongy layer is necessary for maintaining the moisture environment on the wound surface. The optimal weight ratio of HA/Col is determined by considering the following characteristics: mechanical properties for handling, cell viability after thawing, potency of vascular endothelial growth factor (VEGF) release after thawing, efficacy of wound healing, and manufacturing cost. This study is designed to investigate the physical properties for handling, the growth behavior of fibroblasts on the spongy matrix, and the quantitative analysis of VEGF released from fibroblasts in the fresh or cryopreserved CDS. The results of this study suggest that a CDS composed of Col spongy matrix alone has the highest potency in regard to the release of VEGF. However, taking into account the manufacturing cost, coupled with the potency of VEGF release, a two-layered sponge of HA and Col with a weight ratio of 5/2 is very promising for commercial application.
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影响因子：
2
作者：
WEIGEL, PH;FULLER, GM;LEBOEUF, RD
通讯作者：
LEBOEUF, RD
影响因子：
3.2
作者：
REPESH, LA;FITZGERALD, TJ;FURCHT, LT
通讯作者：
FURCHT, LT
人真皮成纤维细胞前列腺素 E2 合成的子系异质性。
影响因子：
--
作者：
Korn,JH
通讯作者：
Korn,JH
成纤维细胞对单核细胞衍生介质反应的克隆异质性。
影响因子：
--
作者：
Korn,JH;Torres,D;Downie,E
通讯作者：
Downie,E
影响因子：
2.2
作者：
REGAN, MC;KIRK, SJ;BARBUL, A
通讯作者：
BARBUL, A