CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation.
CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation.
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DOI：
10.4049/jimmunol.1102885
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发表时间：
2012-05-01
期刊：
影响因子：
--
通讯作者：
Shlomchik MJ
中科院分区：
文献类型：
--
作者：
Good-Jacobson KL;Song E;Anderson S;Sharpe AH;Shlomchik MJ
尽管调节这些长寿群体形成的机制尚不清楚，但在高亲和力长寿浆细胞（PC）和记忆B细胞的产生中，生殖中心（GC）B细胞和T滤泡辅助（TFH）细胞相互作用。由于CD 80是人类和小鼠记忆B细胞共有的少数标志物之一，我们研究了其在GC、记忆细胞和PC发育中的作用。在CD 80缺陷小鼠中，与B6对照相比，免疫后产生的长寿命PC较少。一致地，在GC收缩期，CD 80的缺乏导致凋亡的GC B细胞增加。与B6相比，CD 80 −/−小鼠的TFH较少，残留的TFH细胞未能成熟，ICOS和PD-1表达减少，IL-21 mRNA合成减少。混合骨髓嵌合体表现出B细胞对正常TFH和PC发育的CD 80表达的内在要求。因此，B细胞表达CD 80在调节早期和晚期GC反应中的B-T相互作用中起关键作用。这反过来又导致生产长寿命PC的能力受损。这些数据为GC和AFC的发育以及CD 80在体液免疫中的功能提供了新的见解。
Germinal center (GC) B cells and T follicular helper (TFH) cells interact in the production of high-affinity long-lived plasma cells (PCs) and memory B cells, though the mechanisms regulating the formation of these long-lived populations remain unclear. Because CD80 is one of the few markers shared by human and murine memory B cells, we investigated its role in the development of GCs, memory cells and PCs. In CD80-deficient mice, fewer long-lived PCs were generated upon immunization, compared to B6 controls. In concert, the absence of CD80 resulted in an increase in apoptotic GC B cells during the contraction phase of the GC. CD80−/− mice had fewer TFH compared to B6, and residual TFH cells failed to mature, with decreased ICOS and PD-1 expression and decreased synthesis of IL-21 mRNA. Mixed bone marrow chimeras demonstrated a B cell-intrinsic requirement for CD80 expression for normal TFH and PC development. Therefore, B cell expression of CD80 plays a critical role in regulating B-T interactions in both early and late GC responses. This, in turn, results in impaired ability to produce long-lived PCs. These data provide new insights into the development of GCs and AFCs and the functions of CD80 in humoral immunity.
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影响因子：
4.4
作者：
CHEN, JZ;TROUNSTINE, M;HUSZAR, D
通讯作者：
HUSZAR, D
影响因子：
56.9
作者：
Allen, Christopher D. C.;Okada, Takaharu;Cyster, Jason G.
通讯作者：
Cyster, Jason G.
影响因子：
32.4
作者：
Choi YS;Kageyama R;Eto D;Escobar TC;Johnston RJ;Monticelli L;Lao C;Crotty S
通讯作者：
Crotty S
DOI：
10.1084/jem.20062571
发表时间：
2007-09-03
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Anderson SM;Tomayko MM;Ahuja A;Haberman AM;Shlomchik MJ
通讯作者：
Shlomchik MJ
影响因子：
30.5
作者：
Dogan, Ismail;Bertocci, Barbara;Weill, Jean-Claude
通讯作者：
Weill, Jean-Claude