Detection of autoantibodies against recombinant desmoglein 1 and 3 molecules in patients with pemphigus vulgaris: correlation with disease extent at the time of diagnosis and during follow-up.
Detection of autoantibodies against recombinant desmoglein 1 and 3 molecules in patients with pemphigus vulgaris: correlation with disease extent at the time of diagnosis and during follow-up.
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DOI：
10.1155/2009/187864
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发表时间：
2009
影响因子：
--
通讯作者：
Alaibac M
中科院分区：
文献类型：
--
作者：
Belloni-Fortina A;Faggion D;Pigozzi B;Peserico A;Bordignon M;Baldo V;Alaibac M
最近获得的天疱疮抗原的cDNA克隆允许生产重组桥粒芯糖蛋白1和桥粒芯糖蛋白3分子，并开发了ELISA方法来确定针对它们的抗体水平。本研究的目的是确定20例寻常型天疱疮患者在诊断时和随访期间自身抗体水平与粘膜和皮肤病变程度之间的关系。为了通过ELISA检测自身抗体，我们使用表达与整个胞外桥粒芯糖蛋白1和桥粒芯糖蛋白3结构域重叠的序列的重组蛋白。我们发现，在存在粘膜病变的情况下，粘膜受累的范围与桥粒芯糖蛋白1和桥粒芯糖蛋白3的自身抗体滴度之间存在相关性，而在存在皮肤病变的情况下，皮肤病变的范围与桥粒芯糖蛋白3的自身抗体滴度之间存在统计学显著相关性，但与桥粒芯糖蛋白1无关。一个不可忽略的病人数显示的桥粒芯蛋白3自身抗体滴度的变化，这并不相关的皮肤和粘膜受累的严重程度。分析针对桥粒芯糖蛋白1的自身抗体滴度获得了类似的结果。总之，我们认为，利用重组桥粒芯糖蛋白1和桥粒芯糖蛋白3的ELISA应谨慎使用，以监测疾病的严重程度和对治疗的反应，虽然它仍然是一个高特异性的测试天疱疮的初步诊断和鉴定这种情况的临床表型的变化。
The recent availability of cDNA clones for pemphigus antigens has allowed the production of recombinant desmoglein 1 and desmoglein 3 molecules and the development of an ELISA approach in order to determine levels of antibodies to them. The aim of the study was to determine the relationship between autoantibodies levels and the extent of both mucosal and skin lesions in 20 patients with pemphigus vulgaris at the time of diagnosis and during follow-up. For the detection of autoantibodies by ELISA we used the recombinant proteins expressing overlapping sequences with the entire extracellular desmoglein 1 and desmoglein 3 domains. We showed that in presence of mucosal lesions there was a correlation between extension of mucosal involvement and autoantiboidies titres against both desmoglein 1 and desmoglein 3, whereas in presence of skin lesions there was a statistically significant correlation between extension of skin lesions and autoantibodies titres against desmoglein 3, but not against desmoglein 1. A not negligible number of patients showed variations of the desmoglein 3 autoantibodies titre which did not correlate with the severity of both cutaneous and mucosal involvement. Similar results were obtained analyzing autoantibodies titres against desmoglein 1. In conclusion, we believe that the utilization of recombinant desmoglein 1 and desmoglein 3 proteins by ELISA should be used with caution to monitor disease severity and response to therapy, although it remains a high specific test for the initial diagnosis of pemphigus and the identification of a change in the clinical phenotype of this condition.
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影响因子：
4.1
作者：
Harman, KE;Gratian, MJ;Black, MM
通讯作者：
Black, MM
DOI：
10.1016/s0190-9622(99)70183-0
发表时间：
1999-02-01
影响因子：
13.8
作者：
Amagai, M;Tsunoda, K;Nishikawa, T
通讯作者：
Nishikawa, T
影响因子：
--
作者：
Abasq, Claire;Mouquet, Hugo;Joly, Pascal
通讯作者：
Joly, Pascal
影响因子：
10.3
作者：
Harman, KE;Seed, PT;Black, MM
通讯作者：
Black, MM
影响因子：
3.6
作者：
Yeh, S W;Ahmed, Babar;Razzaque Ahmed, A
通讯作者：
Razzaque Ahmed, A