Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor.
Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor.
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DOI：
10.1038/ncomms2062
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发表时间：
2012
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
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成熟/M期促进因子是真核细胞中M期的通用诱导剂。目前公认 M 期促进因子与激酶周期蛋白 B–Cdk1 相同。在这里，我们表明细胞周期蛋白 B–Cdk1 和 M 期促进因子实际上并不是同义词。相反，M 期促进因子至少包含两个基本成分：细胞周期蛋白 B–Cdk1 和另一种激酶 Greatwall 激酶。在没有 Greatwall 激酶的情况下，即使细胞周期蛋白 B-Cdk1 完全活跃，卵母细胞细胞质中也检测不到 M 相促进因子，而当重新添加 Greatwall 激酶时，M 相促进因子活性恢复。虽然单独过量的细胞周期蛋白B-Cdk1（而不是单独的长城激酶）可以诱导核膜破裂，纺锤体组装失败。添加 Greatwall 激酶大大减少了核膜分解所需的细胞周期蛋白 B-Cdk1 的量，从而形成具有对齐染色体的纺锤体。因此，M 期促进因子是一个系统，由指导有丝分裂进入的一种激酶（细胞周期蛋白 B–Cdk1）和抑制与细胞周期蛋白 B–Cdk1 对抗的蛋白磷酸酶 2A-B55 的第二种激酶（Greatwall 激酶）组成。 Cyclin B–Cdk1 被认为是驱动进入细胞周期 M 期的促进因子的同义词。在这里，长城激酶被证明是进入 M 期时核膜分解和纺锤体组装所必需的，这表明它也是 M 期促进因子的一部分。
Maturation/M-phase-promoting factor is the universal inducer of M-phase in eukaryotic cells. It is currently accepted that M-phase-promoting factor is identical to the kinase cyclin B–Cdk1. Here we show that cyclin B–Cdk1 and M-phase-promoting factor are not in fact synonymous. Instead, M-phase-promoting factor contains at least two essential components: cyclin B–Cdk1 and another kinase, Greatwall kinase. In the absence of Greatwall kinase, the M-phase-promoting factor is undetectable in oocyte cytoplasm even though cyclin B–Cdk1 is fully active, whereas M-phase-promoting factor activity is restored when Greatwall kinase is added back. Although the excess amount of cyclin B–Cdk1 alone, but not Greatwall kinase alone, can induce nuclear envelope breakdown, spindle assembly is abortive. Addition of Greatwall kinase greatly reduces the amount of cyclin B–Cdk1 required for nuclear envelope breakdown, resulting in formation of the spindle with aligned chromosomes. M-phase-promoting factor is thus a system consisting of one kinase (cyclin B–Cdk1) that directs mitotic entry and a second kinase (Greatwall kinase) that suppresses the protein phosphatase 2A-B55 which opposes cyclin B–Cdk1. Cyclin B–Cdk1 is thought to be synonymous with the promoting factor that drives entry into M-phase of the cell cycle. Here, Greatwall kinase is shown to be required for the breakdown of the nuclear envelope and the assembly of the spindle on entry into M-phase, suggesting that it too is a part of the M-phase-promoting factor.
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影响因子：
11.4
作者：
Hagting, A;Karlsson, C;Pines, J
通讯作者：
Pines, J
影响因子：
4
作者：
Abe, Yusuke;Okumura, Eiichi;Kishimoto, Takeo
通讯作者：
Kishimoto, Takeo
影响因子：
--
作者：
MASUI, Y
通讯作者：
MASUI, Y
DOI：
10.1073/pnas.95.8.4392
发表时间：
1998-04-14
影响因子：
11.1
作者：
Iwashita, J;Hayano, Y;Sagata, N
通讯作者：
Sagata, N
影响因子：
3.3
作者：
Castilho, Priscila V.;Williams, Byron C.;Goldberg, Michael L.
通讯作者：
Goldberg, Michael L.