Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates.
Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates.
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DOI：
10.1007/s00436-013-3297-3
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发表时间：
2013-04
影响因子：
2
通讯作者：
Ohashi K
中科院分区：
文献类型：
--
作者：
Mekata H;Konnai S;Mingala CN;Abes NS;Gutierrez CA;Dargantes AP;Witola WH;Inoue N;Onuma M;Murata S;Ohashi K
近年来，菲律宾出现高致病性伊氏锥虫菌株，导致畜牧业生产遭受重大损失。在这项研究中，我们从菲律宾受感染的水牛中分离出伊氏锥虫，并利用小鼠和牛分析了它们的毒力。总共分离出10株伊氏锥虫。使用小鼠对每种菌株的毒力进行评估，显示菌株之间在潜伏期、寄生虫血症水平和受感染动物的存活时间方面存在显着差异。在感染高致病性伊氏锥虫的小鼠中，感染后 8 天观察到过度炎症的迹象，例如明显的脾肿大和白细胞数量增加超过 6 倍。为了研究牛体内寄生虫菌株（菲律宾常见的伊氏锥虫宿主）的毒力，牛被伊氏锥虫分离株感染，这些分离株在小鼠中表现出高毒力和低毒力。发现寄生虫的生长速度和潜伏期的长度与在小鼠中观察到的各菌株相似。感染高致病性菌株的牛出现贫血，白细胞计数显着下降。为了确定病理变化的原因，我们分析了贫血感染牛中炎症细胞因子的表达水平并观察到肿瘤坏死因子-α的上调。我们的研究结果表明，菲律宾伊氏锥虫流行的特点是伊氏锥虫菌株对牛的致病性从低到极高不等。
In recent years, the emergence of highly pathogenic Trypanosoma evansi strains in the Philippines has resulted in substantial losses in livestock production. In this study, we isolated T. evansi from infected-water buffaloes in the Philippines and analyzed their virulence using mice and cattle. A total of 10 strains of T. evansi were isolated. Evaluation of the virulence of each strain using mice depicted significant differences among the strains in the prepatent period, the level of parasitemia, and the survival time of the infected animals. In mice infected with the highly pathogenic T. evansi, signs of excessive inflammation such as marked splenomegaly and increase more than 6-fold in the number of leukocytes were observed at 8 days post-infection. To study the virulence of the parasite strains in cattle (which are the common T. evansi hosts in Philippines), cattle were infected with the T. evansi isolates that showed high and low virulence in mice. The rate of parasite growth and the length of the prepatent periods were found to be similar to those observed in mice for the respective strains. The cattle infected with the highly pathogenic strain developed anemia and a marked decrease in leukocyte counts. To determine the cause of the pathological changes, we analyzed the expression levels of inflammatory cytokines and observed up-regulation of tumor necrosis factor-α in anemic infected cattle. Our findings suggest that the epidemic of T. evansi in the Philippines is characterized by T. evansi strains with varying virulences from low to very high pathogenicity in cattle.
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影响因子：
6.4
作者：
Magez, S;Radwanska, M;De Baetselier, P
通讯作者：
De Baetselier, P
影响因子：
2.1
作者：
LANHAM, SM;GODFREY, DG
通讯作者：
GODFREY, DG
影响因子：
2
作者：
Onah, DN;Hopkins, J;Luckins, AG
通讯作者：
Luckins, AG
DOI：
10.1083/jcb.137.3.715
发表时间：
1997-05-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Magez S;Geuskens M;Beschin A;del Favero H;Verschueren H;Lucas R;Pays E;de Baetselier P
通讯作者：
de Baetselier P
影响因子：
3.1
作者：
Magez, S;Radwanska, M;De Baetselier, P
通讯作者：
De Baetselier, P