Endothelial Cells: Co-culture Spheroids.

Endothelial Cells: Co-culture Spheroids.
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内皮细胞:共培养球体。

DOI:
10.1007/978-1-0716-0916-3_5
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发表时间:
2021
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Kanczler JM
Kanczler JM
中科院分区:
--
文献类型:
--
作者:
Kanczler JM

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功能性血管系统的发育和维持是组织生长和再生的许多方面的关键功能。血管内皮细胞在体外共培养球体是自组织的细胞复合物,具有重演三维组织微环境的能力。这些球体测试平台旨在更好地了解功能组织的机制以及新的治疗药物如何驱动这些3D共培养过程。在这里,我们描述了直接细胞-细胞3D内皮共培养球体方法,以检查血管细胞和周围天然组织细胞在球体内血管网络形成中的生理空间生长和细胞-细胞相互作用以及再生组织的潜力。
The development and maintenance of a functioning vascular system is a critical function for many aspects of tissue growth and regeneration. Vascular endothelial cell in vitro co-culture spheroids are self-organized cell composites that have the capacity to recapitulate the three-dimensional tissue microenvironment. These spheroid testing platforms aim to better understand the mechanisms of functional tissue and how new therapeutic agents can drive these 3D co-culture processes. Here we describe direct cell–cell 3D endothelial co-culture spheroid methods, to examine the physiological spatial growth and cell–cell interaction of vascular cells and surrounding native tissue cells in the formation of vascular networks within spheroids and the potential to regenerate tissue.
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影响因子: 4.6
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