Distinct glutamatergic and GABAergic subsets of hypothalamic pro-opiomelanocortin neurons revealed by in situ hybridization in male rats and mice.
Distinct glutamatergic and GABAergic subsets of hypothalamic pro-opiomelanocortin neurons revealed by in situ hybridization in male rats and mice.
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DOI：
10.1002/cne.23350
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发表时间：
2013-10-01
影响因子：
2.5
通讯作者：
Lechan, Ronald M.
中科院分区：
文献类型：
--
作者：
Wittmann, Gabor;Hrabovszky, Erik;Lechan, Ronald M.
下丘脑的前鸦片黑素皮质素（POMC）和刺痛酶相关蛋白（AGRP）神经元调节能量稳态和代谢的各个方面。POMC和AGRP神经元分别对其共同下游神经元上的黑素皮质素受体产生痛苦和拮抗作用。然而，尚不清楚它们是否也通过氨基酸递质相互刺激和抑制相同的神经元。虽然AGRP神经元主要是gaba能神经元，但令人惊讶的是，只有一小部分POMC神经元被发现是谷氨酸能神经元，而更大的亚群是gaba能神经元。为了进一步研究POMC神经元的氨基酸表型，我们利用原位杂交技术研究了大鼠和小鼠POMC神经元中谷氨酸能标记物2型囊泡谷氨酸转运体（VGLUT2）和GABA合成酶谷氨酸脱羧酶67 （GAD67）的mRNA表达。在大鼠中，大约58%的POMC神经元被标记为VGLUT2, 37%被标记为GAD67 mRNA。在小鼠中，大约43%的POMC神经元含有VGLUT2, 54%含有GAD67 mRNA。在这两个物种中，在吻侧和中水平的POMC细胞组中观察到一个突出的中外侧分布模式，VGLUT2-POMC神经元在外侧部分占主导地位，GAD67-POMC神经元在内侧部分占主导地位。这些数据表明，谷氨酸能细胞和gaba能细胞在POMC神经元中都有相当数量的存在。它们的谷氨酸能或gaba能表型可能代表POMC细胞群中的主要功能分裂。
Pro-opiomelanocortin (POMC) and agouti-related protein (AGRP) neurons in the hypothalamus regulate various aspects of energy homeostasis and metabolism. POMC and AGRP neurons, respectively, agonize and antagonize melanocortin receptors on their common downstream neurons. However, it is unknown whether they also reciprocally stimulate and inhibit the same neurons by amino acid transmitters. Whereas AGRP neurons are mostly GABAergic, surprisingly, only a small population of POMC neurons has been found to be glutamatergic, and a significantly larger subpopulation to be GABAergic. To further examine amino acid phenotypes of POMC neurons, we studied mRNA expression for the glutamatergic marker, type 2 vesicular glutamate transporter (VGLUT2), and the GABA synthetic enzyme, glutamic acid decarboxylase 67 (GAD67), in POMC neurons of both rats and mice by using in situ hybridization techniques. In rats, approximately 58% of POMC neurons were labeled for VGLUT2 and 37% for GAD67 mRNA. In mice, approximately 43% of POMC neurons contained VGLUT2, and 54% contained GAD67 mRNA. In both species, a prominent mediolateral distribution pattern was observed at rostral and mid levels of the POMC cell group with VGLUT2–POMC neurons dominating in lateral portions and GAD67–POMC neurons in medial portions. These data demonstrate that both glutamatergic and GABAergic cells are present in comparably significant numbers among POMC neurons. Their glutamatergic or GABAergic phenotype may represent a major functional division within the POMC cell group.
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影响因子：
16.2
作者：
Elias, CF;Aschkenasi, C;Elmquist, JK
通讯作者：
Elmquist, JK
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
DOI：
10.1073/pnas.1016785108
发表时间：
2011-01-04
影响因子：
11.1
作者：
Ghamari-Langroudi, Masoud;Srisai, Dollada;Cone, Roger D.
通讯作者：
Cone, Roger D.
DOI：
10.1523/jneurosci.3770-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hentges ST;Otero-Corchon V;Pennock RL;King CM;Low MJ
通讯作者：
Low MJ
影响因子：
2.5
作者：
ESCLAPEZ, M;TILLAKARATNE, NJK;HOUSER, CR
通讯作者：
HOUSER, CR