Associations between patient clinical characteristics and the presence of cytomegalovirus DNA in the bronchoalveolar lavage fluid of children with recurrent wheezing.
Associations between patient clinical characteristics and the presence of cytomegalovirus DNA in the bronchoalveolar lavage fluid of children with recurrent wheezing.
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DOI：
10.1186/s12879-018-3345-9
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发表时间：
2018-09-10
影响因子：
3.7
通讯作者：
Ji W
中科院分区：
文献类型：
--
作者：
Sun H;Li S;Yan Y;Chen Z;Wang Y;Hao C;Ji W
本研究旨在调查反复喘息儿童支气管肺泡灌洗液（BALF）中巨细胞病毒（CMV）DNA的发生率，并确定与某些患者临床特征的相关性。在这项横断面研究中，苏州大学医院收治的反复喘息（每年喘息≥ 4次）的儿科患者（年龄< 36个月）入组研究。采用实时荧光定量PCR检测BALF中巨细胞病毒DNA。定量血液免疫球蛋白和T淋巴细胞亚群。比较支气管肺泡灌洗液（BALF）CMV DNA阳性与阴性患者的临床特点。使用Mann-Whitney U检验比较组间非正态分布的连续变量。使用卡方检验进行频率分布的比较。采用斯皮尔曼等级相关系数评价CMV DNA拷贝数与连续变量之间的相关性。共有111例年龄为4 - 36个月（中位14.0（IQR 8.0-22.0）个月）的患者入组研究。在51.4%的复发性喘息患者（n = 111）中检测到巨细胞病毒DNA，在年龄12至36个月且改良哮喘预测指数（mAPI）阳性的患者中更为普遍。（n = 38，中位23.5（IQR 19.7-31.2）个月），高于mAPI阴性的相同年龄组（n = 25，中位数15.0（IQR 13.0-19.0）个月）（57.9% vs. 20.0%，p = 0.003）。支气管肺泡灌洗液CMV DNA拷贝数[中位数7560（IQR 1200- 71，150）拷贝/mL]与住院时间呈正相关（r = 0.33，p = 0.013），与患者年龄呈负相关（r =-0.41，p = 0.002）和BALF嗜酸性粒细胞百分比（r =-0.38，p = 0.004）。反复喘息患儿CMV感染或再激活与某些临床特征相关，包括年龄较小和BALF嗜酸性粒细胞水平较低。较高的CMV DNA拷贝数与较长的住院时间相关。需要进一步的研究来解决特异性抗病毒治疗是否对BALF CMV阳性患者有益。
This study aimed to investigate the occurrence of Cytomegalovirus (CMV) DNA in the Bronchoalveolar lavage fluid (BALF) of children with recurrent wheezing and to identify associations with certain patient clinical characteristics. In this cross-sectional study, pediatric patients (age < 36 months) admitted to Soochow University Hospital with recurrent wheezing (≥ 4 episodes of wheezing per year) were enrolled in the study. Cytomegalovirus DNA from their BALF was detected by real-time PCR. Subpopulations of blood immunoglobulins and T lymphocytes were quantified. The clinical characteristics of patients with and without BALF CMV DNA were compared. Comparisons of non-normally distributed continuous variables between groups were made using the Mann-Whitney U-test. Comparisons of frequency distributions were made using the Chi-squared test. Spearman’s rank correlation coefficient was used to evaluate correlations between the number of CMV DNA copies and continuous variables. A total of 111 patients aged 4 to 36 months (median 14.0 (IQR 8.0–22.0) months) were enrolled on to the study. Cytomegalovirus DNA was detected in 51.4% of patients (n = 111) with recurrent wheeze and was more prevalent among those aged 12 to 36 months with a positive modified asthma predictive index (mAPI) (n = 38, median 23.5 (IQR 19.7–31.2) months) than in those of the same age group with a negative mAPI (n = 25, median 15.0 (IQR 13.0–19.0) months) (57.9% vs. 20.0%, p = 0.003). Bronchoalveolar lavage fluid CMV DNA copy number [median 7560 (IQR 1200–71,150) copies/mL] was positively correlated with the duration of hospitalization (r = 0.33, p = 0.013), and negatively correlated with patient age (r = − 0.41, p = 0.002) and the percentage of BALF eosinophils (r = − 0.38, p = 0.004). CMV infection or reactivation in children with recurrent wheeze is associated with certain clinical characteristics, including younger age and lower levels of BALF eosinophils. Higher CMV DNA copy numbers were associated with a longer duration of hospitalization. Further studies are needed to address whether specific antiviral treatment could be beneficial for BALF CMV positive patients.
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影响因子：
12.4
作者：
Kowalski, M. L.;Wardzynska, A.;Paradowska, E.
通讯作者：
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DOI：
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发表时间：
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DOI：
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发表时间：
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期刊：
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作者：
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DOI：
10.1016/j.jaip.2012.10.008
发表时间：
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影响因子：
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作者：
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