Multimodal in vivo recording using transparent graphene microelectrodes illuminates spatiotemporal seizure dynamics at the microscale.
Multimodal in vivo recording using transparent graphene microelectrodes illuminates spatiotemporal seizure dynamics at the microscale.
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DOI：
10.1038/s42003-021-01670-9
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发表时间：
2021-01-29
影响因子：
5.9
通讯作者：
Vitale F
中科院分区：
文献类型：
--
作者：
Driscoll N;Rosch RE;Murphy BB;Ashourvan A;Vishnubhotla R;Dickens OO;Johnson ATC;Davis KA;Litt B;Bassett DS;Takano H;Vitale F
癫痫等神经系统疾病是由大脑网络紊乱引起的。我们治疗这些疾病的能力受到限制，因为我们无法在足够的时间和空间尺度上绘制这些网络，以针对干预措施。目前最好的技术要么是低时间分辨率的大面积采样（如钙成像），要么是高时间分辨率的离散区域记录（如电生理学）。这种限制阻碍了我们理解和干预网络动态异常的能力。在这里，我们提出了一种技术，通过使用透明石墨烯微电极阵列同时记录高带宽微皮质电成像和钙荧光，来绘制急性癫痫发作在体内的发病和时空传播。我们使用非负矩阵分解法整合了两种模式的动态数据特征，以确定癫痫发作和进化的时序时空模式，揭示了癫痫电生理的时间进展如何与癫痫发作核心的空间演变相关联。这种对多模态数据的综合分析揭示了在急性发作的空间和时间进展中隐藏的状态转变。这里展示的技术可能使未来有针对性的治疗干预和癫痫发作和进化过程中局部电路动力学的新型空间嵌入模型成为可能。Driscoll， Rosch等人设计了透明石墨烯表面探针，实现了小鼠癫痫发作的同时电生理记录和钙成像。该方法可用于研究癫痫发作和演化过程中的电路动力学，具有较高的时间和空间分辨率。
Neurological disorders such as epilepsy arise from disrupted brain networks. Our capacity to treat these disorders is limited by our inability to map these networks at sufficient temporal and spatial scales to target interventions. Current best techniques either sample broad areas at low temporal resolution (e.g. calcium imaging) or record from discrete regions at high temporal resolution (e.g. electrophysiology). This limitation hampers our ability to understand and intervene in aberrations of network dynamics. Here we present a technique to map the onset and spatiotemporal spread of acute epileptic seizures in vivo by simultaneously recording high bandwidth microelectrocorticography and calcium fluorescence using transparent graphene microelectrode arrays. We integrate dynamic data features from both modalities using non-negative matrix factorization to identify sequential spatiotemporal patterns of seizure onset and evolution, revealing how the temporal progression of ictal electrophysiology is linked to the spatial evolution of the recruited seizure core. This integrated analysis of multimodal data reveals otherwise hidden state transitions in the spatial and temporal progression of acute seizures. The techniques demonstrated here may enable future targeted therapeutic interventions and novel spatially embedded models of local circuit dynamics during seizure onset and evolution. Driscoll, Rosch et al. design transparent graphene-based surface probes to achieve simultaneous electrophysiological recording and calcium imaging of epileptic seizures in mice. This method could be used to investigate circuit dynamics during seizure onset and evolution with high temporal and spatial resolution.
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影响因子：
5.7
作者：
Goodfellow, Marc;Schindler, Kaspar;Baier, Gerold
通讯作者：
Baier, Gerold
DOI：
10.1039/c5tb00108k
发表时间：
2015-07-07
期刊：
Journal of materials chemistry. B
影响因子：
--
作者：
Kozai TD;Vazquez AL
通讯作者：
Vazquez AL
影响因子：
5.6
作者：
Bragin, A;Wilson, CL;Engel, T
通讯作者：
Engel, T
影响因子：
25
作者：
Dworkin, Jordan D.;Linn, Kristin A.;Bassett, Danielle S.
通讯作者：
Bassett, Danielle S.
影响因子：
5.8
作者：
Kim, Hyunsoo;Park, Haesun
通讯作者：
Park, Haesun