Systemically delivered insulin-like growth factor-I enhances mesenchymal stem cell-dependent fracture healing.
Systemically delivered insulin-like growth factor-I enhances mesenchymal stem cell-dependent fracture healing.
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DOI：
10.3109/08977194.2012.683188
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Spagnoli A
中科院分区：
文献类型：
--
作者：
Myers TJ;Yan Y;Granero-Molto F;Weis JA;Longobardi L;Li T;Li Y;Contaldo C;Ozkan H;Spagnoli A
在这项研究中，我们研究了重组胰岛素样生长因子(IGF)- 1皮下传递与原代培养的骨髓间充质干细胞（MSC）移植对骨折修复的有效性。我们发现，与未治疗或单独接受IGF-I + MSC治疗的小鼠相比，接受IGF-I + MSC治疗的稳定胫骨骨折小鼠的骨折痂体积增加。在评估骨痂组织成分时，我们发现IGF-I + MSC受体的软组织和新生骨组织体积显著增加。组织学和原位杂交分析证实，IGF-I + MSC受体中新形成骨的特征性增加，并且愈合主要通过软骨内成骨进行。生物力学测试表明，软组织和新生骨组织体积的增加与力和韧性的增加相关。总之，骨髓间充质干细胞移植同时全身输送IGF-I可改善骨折修复，这表明IGF-I +骨髓间充质干细胞可能是骨折修复不足患者的一种新的治疗方法。
In this study, we examined the effectiveness of systemic subcutaneous delivery of recombinant Insulin-like growth factor (IGF)-I concurrently with primary cultured bone marrow-derived mesenchymal stem cell (MSC) transplant on fracture repair. We found that the fracture callus volume increased in mice with a stabilized tibia fracture that received IGF-I + MSC when compared with that in either untreated or MSC alone treated mice. In evaluating the callus tissue components, we found that the soft and new bone tissue volumes were significantly increased in IGF-I + MSC recipients. Histological and in-situ hybridization analyses confirmed a characteristic increase of newly forming bone in IGF-I + MSC recipients and that healing progressed mostly through endochondral ossification. The increase in soft and new bone tissue volumes correlated with increased force and toughness as determined by biomechanical testing. In conclusion, MSC transplant concurrent with systemic delivery of IGF-I improves fracture repair suggesting that IGF-I + MSC could be a novel therapeutic approach in patients who have inadequate fracture repair.
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影响因子：
5.2
作者：
Granero-Molto, Froilan;Myers, Timothy J.;Weis, Jared A.;Longobardi, Lara;Li, Tieshi;Yan, Yun;Case, Natasha;Rubin, Janet;Spagnoli, Anna
通讯作者：
Spagnoli, Anna
DOI：
10.1097/phm.0b013e31818e002d
发表时间：
2009-02-01
影响因子：
3
作者：
Di Monaco, Marco;Vallero, Fulvia;Cavanna, Alberto
通讯作者：
Cavanna, Alberto
影响因子：
6.2
作者：
Fowlkes, John L.;Thrailkill, Kathryn M.;Lumpkin, Charles K., Jr.
通讯作者：
Lumpkin, Charles K., Jr.
影响因子：
158.5
作者：
GULER, HP;ZAPF, J;FROESCH, ER
通讯作者：
FROESCH, ER
影响因子：
5.8
作者：
Langlois, JA;Rosen, CJ;Kiel, DP
通讯作者：
Kiel, DP