Overexpression of the tonoplast sugar transporter CmTST2 in melon fruit increases sugar accumulation.
Overexpression of the tonoplast sugar transporter CmTST2 in melon fruit increases sugar accumulation.
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甜瓜果实中液泡膜糖转运蛋白 CmTST2 的过度表达增加了糖的积累
DOI：
10.1093/jxb/erx440
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发表时间：
2018-01-23
影响因子：
6.9
通讯作者：
Bie Z
中科院分区：
文献类型：
--
作者：
Cheng J;Wen S;Xiao S;Lu B;Ma M;Bie Z
CmTST2是甜瓜果实发育过程中高表达的一种糖转运蛋白，其过表达也会增加草莓和黄瓜果实中糖的积累。水果是人类饮食的重要组成部分，含糖量是评价水果品质的主要标准。甜瓜果实在发育过程中积累了较高的糖浓度；然而，这些糖被运输到果细胞液泡中储存的机制尚不清楚。本研究从甜瓜植物中分离到了三种糖转运蛋白CmTST1、CmTST2和CmTST3。亚细胞定位分析表明，这些蛋白均以细胞质为靶点，空间表达和启动子gus活性分析表明，它们在植物中具有不同的表达模式。RT-PCR和qRT-PCR结果表明，CmTST2在甜瓜果实发育过程中表达量最高。通过组织化学和免疫组织化学定位实验确定了CmTST2在果实中的组织型和细胞型定位，结果表明其转录和翻译均在中果皮和维管细胞中进行。CmTST2基因在草莓果实和黄瓜植株中分别通过瞬时表达和稳定转化过表达，均增加了果实中蔗糖、果糖和葡萄糖的积累。结果表明，CmTST2在甜瓜果实糖积累中起重要作用。
CmTST2 is a tonoplast sugar transporter that is highly expressed during melon fruit development, and its overexpression also increases sugar accumulation in strawberry and cucumber fruits. Fruits are an important part of the human diet and sugar content is a major criterion used to evaluate fruit quality. Melon fruit accumulate high sugar concentrations during their development; however, the mechanism through which these sugars are transported into the vacuoles of fruit cells for storage remains unclear. In this study, three tonoplast sugar transporters (TSTs), CmTST1, CmTST2, and CmTST3, were isolated from melon plants. Analysis of subcellular localization revealed that all these proteins were targeted to the tonoplast, and evaluation of spatial expression and promoter-GUS activity indicated that they had different expression patterns in the plant. RT-PCR and qRT-PCR results indicated that CmTST2 exhibited the highest expression level among the TST isoforms during melon fruit development. Histochemical and immunohistochemistry localization experiments were performed to identify the tissue- and cell-type localization of CmTST2 in the fruit, and the results indicated that both its transcription and translation were in the mesocarp and vascular cells. Overexpressing the CmTST2 gene in strawberry fruit and cucumber plants by transient expression and stable transformation, respectively, both increased sucrose, fructose, and glucose accumulation in the fruit. The results indicate that CmTST2 plays an important role in sugar accumulation in melon fruit.
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影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
9.4
作者：
Cho, Jung-Il;Burla, Bo;Jeon, Jong-Seong
通讯作者：
Jeon, Jong-Seong
影响因子：
4.1
作者：
Bartolozzi, F;Bertazza, G;Cristoferi, G
通讯作者：
Cristoferi, G
DOI：
10.21273/jashs.132.5.704
发表时间：
2007-09-01
影响因子：
1.9
作者：
Burger, Yosef;Schaffer, Arthur A.
通讯作者：
Schaffer, Arthur A.
影响因子：
7.2
作者：
Chen, Hsin-Yi;Huh, Jung-Hyun;Guo, Woei-Jiun
通讯作者：
Guo, Woei-Jiun