Hydroxyapatite nanoparticle-modified porous bone grafts with improved cell attachment.
Hydroxyapatite nanoparticle-modified porous bone grafts with improved cell attachment.
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DOI：
10.1039/d3tb01839c
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发表时间：
2023-11-15
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影响因子：
--
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中科院分区：
文献类型：
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乳液模板泡沫已显示出作为可注射骨移植物的前景;然而，使用表面活性剂作为乳化剂导致相对较小的孔隙并阻碍细胞附着。羟基磷灰石纳米粒子作为一种替代稳定剂，以解决这些限制。为此，首先用肉豆蔻酸修饰羟基磷灰石纳米颗粒以产生适当的疏水性平衡，从而稳定新戊二醇二丙烯酸酯和1，4-丁二硫醇的油包水乳液。原位表面改性与羟基磷灰石所得泡沫被证实与元素映射和透射电子显微镜。纳米颗粒稳定的泡沫显示出相对于表面活性剂稳定的泡沫（23 ± 11%）改善的人间充质干细胞活力（91 ± 5%）。尽管孔径适合骨移植应用（115 ± 71 μm），但泡沫缺乏细胞浸润所需的互连结构。我们假设，表面活性剂和纳米颗粒的共稳定方法可用于实现互连孔，同时保持改善的细胞附着和更大的孔径。研究了羟基磷灰石纳米颗粒和表面活性剂浓度的范围，以确定对微结构和细胞行为的影响。通过平衡这些相互作用，确定了具有大的互连孔（108 ± 67 μm）并改善了细胞活力和附着的共稳定泡沫。然后将共稳定泡沫作为可注射骨移植物进行评价，包括网络形成、与骨的微尺度整合、推出强度和压缩性能。总体而言，这项工作表明，原位表面改性与nHA改善细胞附着，同时保留所需的骨移植功能和注射。羟基磷灰石纳米粒子和表面活性剂浓度的聚MIPE支架影响微结构和细胞行为。共稳定的polyMIPE平衡这些相互作用，以实现适合骨移植应用的性能。
Emulsion-templated foams have displayed promise as injectable bone grafts; however, the use of a surfactant as an emulsifier resulted in relatively small pores and impedes cell attachment. Hydroxyapatite nanoparticles were explored as an alternative stabilizer to address these limitations. To this end, hydroxyapatite nanoparticles were first modified with myristic acid to generate the appropriate balance of hydrophobicity to stabilize a water-in-oil emulsion of neopentyl glycol diacrylate and 1,4-butanedithiol. In situ surface modification of the resulting foam with hydroxyapatite was confirmed with elemental mapping and transmission electron microscopy. Nanoparticle-stabilized foams displayed improved human mesenchymal stem cell viability (91 ± 5%) over surfactant-stabilized foams (23 ± 11%). Although the pore size was appropriate for bone grafting applications (115 ± 71 μm), the foams lacked the interconnected architecture necessary for cell infiltration. We hypothesized that a co-stabilization approach with both surfactant and nanoparticles could be used to achieve interconnected pores while maintaining improved cell attachment and larger pore sizes. A range of hydroxyapatite nanoparticle and surfactant concentrations were investigated to determine the effects on microarchitecture and cell behavior. By balancing these interactions, a co-stabilized foam was identified that possessed large, interconnected pores (108 ± 67 μm) and improved cell viability and attachment. The co-stabilized foam was then evaluated as an injectable bone graft including network formation, microscale integration with bone, push out strength, and compressive properties. Overall, this work demonstrated that in situ surface modification with nHA improved cell attachment while retaining desirable bone grafting features and injectability. Hydroxyapatite nanoparticles and surfactant concentration in polyMIPE scaffolds affect microarchitecture and cell behavior. Co-stabilized polyMIPEs balance these interactions to achieve properties suitable for bone grafting applications.
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DOI：
10.1016/j.colsurfa.2004.10.116
发表时间：
2005-02-01
影响因子：
5.2
作者：
Binks, BP;Whitby, CP
通讯作者：
Whitby, CP
影响因子：
3.9
作者：
Binks, BP;Lumsdon, SO
通讯作者：
Lumsdon, SO
DOI：
10.1007/s10856-007-3347-4
发表时间：
2008-06-01
影响因子：
3.7
作者：
dos Santos, E. A.;Farina, M.;Anselme, K.
通讯作者：
Anselme, K.
影响因子：
3.9
作者：
Binks, BP;Lumsdon, SO
通讯作者：
Lumsdon, SO
影响因子：
6.2
作者：
Dikici, Betul Aldemir;Malayeri, Atra;Claeyssens, Frederik
通讯作者：
Claeyssens, Frederik