Potentiality of a triple microRNA classifier: miR-193a-3p, miR-23a and miR-338-5p for early detection of colorectal cancer.
Potentiality of a triple microRNA classifier: miR-193a-3p, miR-23a and miR-338-5p for early detection of colorectal cancer.
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DOI：
10.1186/1471-2407-13-280
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发表时间：
2013-06-08
期刊：
影响因子：
3.8
通讯作者：
Wang CW
中科院分区：
文献类型：
--
作者：
Yong FL;Law CW;Wang CW
MicroRNA (miRNA) 是短的非编码 RNA 分子，充当基因表达的调节因子。循环血液 miRNA 作为癌症生物标志物具有巨大的潜力。本研究的目的是关联组织和血液中 miRNA 的差异表达，以识别早期检测结直肠癌 (CRC) 的生物标志物。该研究分为两个阶段：(I) 使用配对癌组织 (n?=?30) 和血液样本 (CRC，n?=?42；对照，n?=?18) 通过 miRNA 微阵列发现标记。 (II)使用一组独立的配对癌组织(n?=?30)和血液样本(CRC，n?=?70；对照，n?=?32)通过茎环逆转录实时PCR进行标记验证。相关分析由皮尔逊检验确定。应用逻辑回归和受试者工作特征曲线分析来获得 miRNA 的诊断效用。已发现 7 种 miRNA（miR-150、miR-193a-3p、miR-23a、miR-23b、miR-338-5p、miR-342-3p 和 miR-483-3p）在组织和血液样本中存在差异表达。组织和血液中 miR-193a-3p、miR-23a 和 miR-338-5p 水平呈显着正相关。此外，在更晚期阶段检测到这些 miRNA 的表达增加。 miR-193a-3p、miR-23a 和 miR-338-5p 被证明可作为 CRC 检测的分类器，产生的受试者工作特征曲线面积为 0.887（敏感性为 80.0%，特异性为 84.4%，准确性为 83.3%）。循环血液 miRNA 的失调反映了结直肠组织中的失调。 miR-193a-3p、miR-23a 和 miR-338-5p 的三重 miRNA 分类器似乎是早期检测 CRC 的潜在血液生物标志物。
MicroRNAs (miRNAs) are short, non-coding RNA molecules that act as regulators of gene expression. Circulating blood miRNAs offer great potential as cancer biomarkers. The objective of this study was to correlate the differential expression of miRNAs in tissue and blood in the identification of biomarkers for early detection of colorectal cancer (CRC). The study was divided into two phases: (I) Marker discovery by miRNA microarray using paired cancer tissues (n?=?30) and blood samples (CRC, n?=?42; control, n?=?18). (II) Marker validation by stem-loop reverse transcription real time PCR using an independent set of paired cancer tissues (n?=?30) and blood samples (CRC, n?=?70; control, n?=?32). Correlation analysis was determined by Pearson’s test. Logistic regression and receiver operating characteristics curve analyses were applied to obtain diagnostic utility of the miRNAs. Seven miRNAs (miR-150, miR-193a-3p, miR-23a, miR-23b, miR-338-5p, miR-342-3p and miR-483-3p) have been found to be differentially expressed in both tissue and blood samples. Significant positive correlations were observed in the tissue and blood levels of miR-193a-3p, miR-23a and miR-338-5p. Moreover, increased expressions of these miRNAs were detected in the more advanced stages. MiR-193a-3p, miR-23a and miR-338-5p were demonstrated as a classifier for CRC detection, yielding a receiver operating characteristic curve area of 0.887 (80.0% sensitivity, 84.4% specificity and 83.3% accuracy). Dysregulations in circulating blood miRNAs are reflective of those in colorectal tissues. The triple miRNA classifier of miR-193a-3p, miR-23a and miR-338-5p appears to be a potential blood biomarker for early detection of CRC.
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