Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals.
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals.
复制标题
正常体重年轻人中因反复部分睡眠剥夺而导致的肠道微生物群和糖代谢改变
DOI：
10.1016/j.molmet.2016.10.003
复制
发表时间：
2016-12
影响因子：
8.1
通讯作者：
Cedernaes J
中科院分区：
文献类型：
--
作者：
Benedict C;Vogel H;Jonas W;Woting A;Blaut M;Schürmann A;Cedernaes J
人类肠道中微生物群落的变化被认为是促进代谢紊乱的原因，这种紊乱也会在短期睡眠不足（包括胰岛素抵抗）后发生。然而，睡眠不足是否会影响肠道微生物群仍然未知。在一项采用标准化实验室方案（固定进餐时间和运动时间表）的随机受试者内交叉研究中，我们在两种情况下研究了9名体重正常的男性：两晚部分睡眠剥夺（PSD;睡眠时间02：45-07：00 h）和两晚正常睡眠（NS;睡眠时间22：30-07：00 h）。在NS或PSD之前24小时内和两个实验室夜晚之后收集粪便样品。此外，参与者在每次睡眠干预后接受口服葡萄糖耐量测试。微生物群组成分析（V4 16 S rRNA基因测序）显示，PSD两天后与NS两天后相比，个体表现出增加的厚壁菌门：拟杆菌门比例，Coriobacteriaceae和丹毒丝菌科的丰度较高，以及Tenericutes的丰度较低（所有P < 0.05）-以前都与动物或人类模型中的代谢紊乱相关。然而，PSD与NS相比，对β多样性或粪便短链脂肪酸浓度没有影响，PSD与NS相比，空腹和餐后胰岛素敏感性降低（均P < 0.05）。我们的研究结果表明，短期睡眠不足会对人体微生物群产生微妙的影响。观察到的微生物群落的变化在多大程度上导致了睡眠不足的代谢后果，这需要在更大规模和更长时间的睡眠研究中进行进一步调查，以评估睡眠不足如何影响已经代谢受损的个体的微生物群。这可能是睡眠时间短如何影响人类肠道微生物群的第一个结果。两个晚上的短暂睡眠不会显著影响β多样性。厚壁菌门与拟杆菌门的比例受到睡眠不足的显著影响。粪便中的短链脂肪酸水平不会因睡眠时间而改变。睡眠不足后胰岛素抵抗增加与微生物群的改变无关。
Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. In a randomized within-subject crossover study utilizing a standardized in-lab protocol (with fixed meal times and exercise schedules), we studied nine normal-weight men at two occasions: after two nights of partial sleep deprivation (PSD; sleep opportunity 02:45–07:00 h), and after two nights of normal sleep (NS; sleep opportunity 22:30–07:00 h). Fecal samples were collected within 24 h before, and after two in-lab nights, of either NS or PSD. In addition, participants underwent an oral glucose tolerance test following each sleep intervention. Microbiota composition analysis (V4 16S rRNA gene sequencing) revealed that after two days of PSD vs. after two days of NS, individuals exhibited an increased Firmicutes:Bacteroidetes ratio, higher abundances of the families Coriobacteriaceae and Erysipelotrichaceae, and lower abundance of Tenericutes (all P < 0.05) – previously all associated with metabolic perturbations in animal or human models. However, no PSD vs. NS effect on beta diversity or on fecal short-chain fatty acid concentrations was found. Fasting and postprandial insulin sensitivity decreased after PSD vs. NS (all P < 0.05). Our findings demonstrate that short-term sleep loss induces subtle effects on human microbiota. To what extent the observed changes to the microbial community contribute to metabolic consequences of sleep loss warrants further investigations in larger and more prolonged sleep studies, to also assess how sleep loss impacts the microbiota in individuals who already are metabolically compromised. Possibly the first results of how short sleep impacts the human gut microbiota. Two nights of short sleep do not significantly impact beta diversity. The Firmicutes to Bacteroidetes ratio is significantly affected by sleep loss. Fecal short-chain fatty acid levels do not change depending on sleep duration. Increased insulin resistance after sleep loss is unrelated to alterations in the microbiota.
登录
查看更多内容
影响因子：
8.1
作者：
Broussard JL;Devkota S
通讯作者：
Devkota S
DOI：
10.1152/ajpgi.2001.280.2.g273
发表时间：
2001-02-01
影响因子：
4.5
作者：
Keller, J;Gröger, G;Layer, P
通讯作者：
Layer, P
影响因子：
7.7
作者：
Buxton OM;Pavlova M;Reid EW;Wang W;Simonson DC;Adler GK
通讯作者：
Adler GK
影响因子：
8.1
作者：
Cani PD;Knauf C
通讯作者：
Knauf C
DOI：
10.1038/ismej.2014.45
发表时间：
2014-10
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--