Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns.
Evolutionary conservation of a molecular machinery for export and expression of mRNAs with retained introns.
复制标题
DOI：
10.1261/rna.048520.114
复制
发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Hammarskjold ML
中科院分区：
文献类型：
--
作者：
Wang B;Rekosh D;Hammarskjold ML
内含子保留是选择性剪接研究最少的形式之一。通过使用逆转录病毒和其他模型系统，许多年前就已经确定，保留内含子的mRNAs在核质输出和细胞质表达水平上都受到限制。还证明了mRNA中特定的顺式作用元件可以用来绕过这些限制。在这里，我们证明了这些元件之一，构成运输元件(CTE)，首先在逆转录病毒MPMV中被发现，随后在人类Nxf1基因中被发现，是一个高度保守的元件。利用GERP分析，在30种哺乳动物的NXF基因中鉴定出具有很强的一级序列同源性的CTE，预测显示出相同的二级结构。在预测的斑马鱼和腔棘鱼的Nxf1基因中也发现了CTE。斑马鱼Nxf1的CTE与斑马鱼Nxf1和辅因子NXT蛋白一起在人类细胞中有效地实现了带有保留内含子的mRNA的表达。这表明，所有表达带有保留内含子的mRNA的必要功能成分在鱼和人之间都是保守的。
Intron retention is one of the least studied forms of alternative splicing. Through the use of retrovirus and other model systems, it was established many years ago that mRNAs with retained introns are subject to restriction both at the level of nucleocytoplasmic export and cytoplasmic expression. It was also demonstrated that specific cis-acting elements in the mRNA could serve to bypass these restrictions. Here we show that one of these elements, the constitutive transport element (CTE), first identified in the retrovirus MPMV and subsequently in the human NXF1 gene, is a highly conserved element. Using GERP analysis, CTEs with strong primary sequence homology, predicted to display identical secondary structure, were identified in NXF genes from >30 mammalian species. CTEs were also identified in the predicted NXF1 genes of zebrafish and coelacanths. The CTE from the zebrafish NXF1 was shown to function efficiently to achieve expression of mRNA with a retained intron in human cells in conjunction with zebrafish Nxf1 and cofactor Nxt proteins. This demonstrates that all essential functional components for expression of mRNA with retained introns have been conserved from fish to man.
登录
查看更多内容
影响因子：
10.5
作者：
Jin, L;Guzik, BW;Hammarskjöld, ML
通讯作者：
Hammarskjöld, ML
影响因子：
5.3
作者：
Ernst, RK;Bray, M;Hammarskjold, ML
通讯作者：
Hammarskjold, ML
影响因子：
4.8
作者：
Dytrych, L;Sherman, DL;Brophy, PJ
通讯作者：
Brophy, PJ
影响因子：
3.6
作者：
Kurio, Hitoshi;Murayama, Emi;Iida, Hiroshi
通讯作者：
Iida, Hiroshi
DOI：
10.1073/pnas.86.5.1495
发表时间：
1989-03-01
影响因子：
11.1
作者：
FELBER, BK;HADZOPOULOUCLADARAS, M;PAVLAKIS, GN
通讯作者：
PAVLAKIS, GN