COMP-angiopoietin-1 promotes cavernous angiogenesis in a type 2 diabetic rat model.
COMP-angiopoietin-1 promotes cavernous angiogenesis in a type 2 diabetic rat model.
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DOI：
10.3346/jkms.2013.28.5.725
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发表时间：
2013-05
影响因子：
4.5
通讯作者：
Park K
中科院分区：
文献类型：
--
作者：
Kim SO;Lee HS;Ahn K;Park K
软骨寡聚基质蛋白-血管生成素-1（COMP-Ang 1）是一种促进血管新生的促血管生成因子。目的是研究海绵体内注射COMP-Ang 1对糖尿病大鼠模型海绵体血管生成的影响。雄性大冢Long-Evans德岛肥胖（OLETF）大鼠组成实验组（1岁），Long-Evans德岛大冢（LETO）大鼠组成对照组。实验组分为仅溶剂、10 µg COMP-Ang 1和20 µg COMP-Ang 1。将COMP-Ang 1注射到阴茎海绵体中。4周后取大鼠阴茎组织进行免疫组化和Western blot分析。与仅溶剂组相比，COMP-Ang 1组中PECAM-1和VEGF的免疫反应性增加。此外，PECAM-1和VEGF的表达在20 μg Comp Ang-1组中显著增加。在免疫印迹研究中，PECAM-1和VEGF蛋白在OLEFT大鼠中的表达显著低于对照LETO大鼠。然而，在海绵体内注射COMP-Ang 1后，这种表达恢复到对照水平。这些结果表明，海绵体内注射COMP-Ang 1通过在结构上增强海绵体内皮来增强海绵体血管生成。
Cartilage oligomeric matrix protein-angiopoietin-1 (COMP-Ang1) is an angiogenic factor for vascular angiogenesis. The aim was to investigate the effect of an intracavernosal injection of COMP-Ang1 on cavernosal angiogenesis in a diabetic rat model. Male Otsuka Long-Evans Tokushima Fatty (OLETF) rats made up the experimental group (1 yr old) and Long-Evans Tokushima Otsuka (LETO) rats made up the control group. The experimental group was divided into vehicle only, 10 µg COMP-Ang1, and 20 µg COMP-Ang1. COMP-Ang1 was injected into the corpus cavernosum of the penis. After 4 weeks, the penile tissues of the rats were obtained for immunohistochemistry and Western blot analysis. The immunoreactivity of PECAM-1 and VEGF was increased in the COMP-Ang1 group compared with the vehicle only group. Moreover, the expression of PECAM-1 and VEGF was notably augmented in the 20 µg Comp Ang-1 group. In the immunoblotting study, the expression of PECAM-1 and VEGF protein was significantly less in the OLEFT rats than in the control LETO rats. However, this expression was restored to control level after intracavernosal injection of COMP-Ang1. These results show that an intracavernosal injection of COMP-Ang1 enhances cavernous angiogenesis by structurally reinforcing the cavernosal endothelium.
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