Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.
Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion.
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DOI：
10.1673/031.010.10701
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Vendan SE
中科院分区：
文献类型：
--
作者：
Anand AA;Vennison SJ;Sankar SG;Prabhu DI;Vasan PT;Raghuraman T;Geoffrey CJ;Vendan SE
Bombyx mori L.（鳞翅目：蚕蛾科）已被驯化并广泛用于丝绸生产。它以桑葚为食。桑葚主要由果胶、木聚糖、纤维素和淀粉组成。一些降解这些碳水化合物的消化酶可能由肠道细菌产生。从B的消化道中分离到11株。mori，包括革兰氏阳性环状芽孢杆菌和革兰氏阴性普通变形杆菌，肺炎克雷伯氏菌，大肠杆菌，弗氏柠檬酸杆菌，产酸沙雷氏菌，肠杆菌属，荧光假单胞菌，铜绿假单胞菌，气单胞菌，和欧文氏菌（Erwinia sp.）其中，普通青霉（P.vulgaris）、K. pneumoniae、C. freundii，是纤维素和木聚糖分解，荧光假单胞菌和欧文氏菌，果胶酶和K.肺炎杆菌降解淀粉。气单胞菌能够利用CM纤维素和木聚糖。S.芽孢杆菌能够利用三种多糖，包括羧甲基纤维素、木聚糖和果胶。B。circulans能够以不同的功效利用所有四种多糖。B的肠子。桑具有碱性pH值和所有分离的细菌菌株被发现生长和降解多糖在碱性pH值。纤维素分解细菌的数量随着每个龄期的增加。
Bombyx mori L. (Lepidoptera: Bombycidae) have been domesticated and widely used for silk production. It feeds on mulberry leaves. Mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. Some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. Eleven isolates were obtained from the digestive tract of B. mori, including the Gram positive Bacillus circulans and Gram negative Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, Citrobacter freundii, Serratia liquefaciens, Enterobacter sp., Pseudomonas fluorescens, P. aeruginosa, Aeromonas sp., and Erwinia sp.. Three of these isolates, P. vulgaris, K. pneumoniae, C. freundii, were cellulolytic and xylanolytic, P. fluorescens and Erwinia sp., were pectinolytic and K. pneumoniae degraded starch. Aeromonas sp. was able to utilize the CMcellulose and xylan. S. liquefaciens was able to utilize three polysaccharides including CMcellulose, xylan and pectin. B. circulans was able to utilize all four polysaccharides with different efficacy. The gut of B. mori has an alkaline pH and all of the isolated bacterial strains were found to grow and degrade polysaccharides at alkaline pH. The number of cellulolytic bacteria increases with each instar.
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DOI：
10.1016/j.cbpb.2004.07.006
发表时间：
2004-09-01
影响因子：
2.3
作者：
Anand, AAP;Sripathi, K
通讯作者：
Sripathi, K
DOI：
10.1590/s0074-02762007000800005
发表时间：
2007-12-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
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通讯作者：
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影响因子：
2.3
作者：
Murakami, T;Ohkawara, K;Higashi, S
通讯作者：
Higashi, S
影响因子：
1.3
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
OPDENCAMP, HJM