HIV-1 Genetic Diversity and Its Impact on Baseline CD4+T Cells and Viral Loads among Recently Infected Men Who Have Sex with Men in Shanghai, China.
HIV-1 Genetic Diversity and Its Impact on Baseline CD4+T Cells and Viral Loads among Recently Infected Men Who Have Sex with Men in Shanghai, China.
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中国上海最近感染的男男性行为者中 HIV-1 遗传多样性及其对基线 CD4 T 细胞和病毒载量的影响
DOI：
10.1371/journal.pone.0129559
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhong P
中科院分区：
文献类型：
--
作者：
Li X;Xue Y;Cheng H;Lin Y;Zhou L;Ning Z;Wang X;Yu X;Zhang W;Shen F;Zheng X;Gai J;Li X;Kang L;Nyambi P;Wang Y;Zhuang M;Pan Q;Zhuang X;Zhong P
男男性行为者（MSM）中的HIV-1流行已在中国各地蔓延。上海市作为男男性接触者的集中地，HIV-1感染率呈持续上升趋势。为了更好地了解HIV-1多样性的动态变化及其对患者基线免疫状态的影响，回顾性分析了2009年1月至2013年12月在上海收集的1265例新感染HIV-1的MSM的基因亚型、CD 4 +T细胞计数和病毒载量。HIV-1系统发育分析显示了广泛的病毒多样性，包括CRF01_AE（62.13%）、CRF07_BC（24.51%）、B亚型（8.06%）、CRF55_01 B（3.24%）、CER67_01 B（0.95%）、CRF68_01 B（0.4%）、CRF08_BC（0.08%）和CRF59_01 B（0.08%）。还鉴定出24种独特的重组形式（URF）（1.98%）。贝叶斯推断分析表明，根据最近共同祖先（tMRCA）的时间，CRF01_AE株（1997）比CRF07_BC株（2001）更早进入上海MSM人群。在CRF01_AE中发现3个流行簇和5个亚簇。CRF01_AE感染者的CD 4 +T细胞计数显著低于CRF07_BC感染者（P<0.01），而病毒载量显著高于CRF07_BC感染者（P<0.01）。年龄>45岁的患者CD 4 +T细胞计数低于<25岁的患者（P <0.05），病毒载量高于<25岁的患者（P<0.05）。本研究揭示了上海地区HIV-1亚型多样性的存在及其对临床结局的显著影响。实时监测HIV-1病毒多样性、聚集性流行动态、患者基线CD 4 +T细胞计数和病毒载量，对监测疾病进展、干预传播、改进抗逆转录病毒治疗策略和疫苗设计具有重要价值。
The HIV-1 epidemic among men who have sex with men (MSM) has been spreading throughout China. Shanghai, a central gathering place for MSM, is facing a continuously increasing incidence of HIV-1 infection. In order to better understand the dynamics of HIV-1 diversity and its influence on patient’s immune status at baseline on diagnosis, 1265 newly HIV-1-infected MSM collected from January 2009 to December 2013 in Shanghai were retrospectively analyzed for genetic subtyping, CD4+T cell counts, and viral loads. HIV-1 phylogenetic analysis revealed a broad viral diversity including CRF01_AE (62.13%), CRF07_BC (24.51%), subtype B (8.06%), CRF55_01B (3.24%), CER67_01B (0.95%), CRF68_01B (0.4%), CRF08_BC (0.08%) and CRF59_01B (0.08%). Twenty-four unique recombination forms (URFs) (1.98%) were identified as well. Bayesian inference analysis indicated that the introduction of CRF01_AE strain (1997) was earlier than CRF07_BC strain (2001) into MSM population in Shanghai based on the time of the most recent common ancestor (tMRCA). Three epidemic clusters and five sub-clusters were found in CRF01_AE. Significantly lower CD4+T cell count was found in individuals infected with CRF01_AE than in those infected with CRF07_BC infection (P<0.01), whereas viral load was significantly higher those infected with CRF01_AE than with CRF07_BC (P<0.01). In addition, the patients with >45 years of age were found to have lower CD4+T cell counts and higher viral loads than the patients with <25 years of age (P<0.05). This study reveals the presence of HIV-1 subtype diversity in Shanghai and its remarkable influence on clinical outcome. A real-time surveillance of HIV-1 viral diversity and phylodynamics of epidemic cluster, patient’s baseline CD4+T cell count and viral load would be of great value to monitoring of disease progression, intervention for transmission, improvement of antiretroviral therapy strategy and design of vaccines.
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DOI：
10.1093/cid/ciq164
发表时间：
2011-02-15
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Kouyos RD;von Wyl V;Yerly S;Böni J;Rieder P;Joos B;Taffé P;Shah C;Bürgisser P;Klimkait T;Weber R;Hirschel B;Cavassini M;Rauch A;Battegay M;Vernazza PL;Bernasconi E;Ledergerber B;Bonhoeffer S;Günthard HF;Swiss HIV Cohort Study
通讯作者：
Swiss HIV Cohort Study
影响因子：
5.4
作者：
Tee, Kok Keng;Pybus, Oliver G.;Takebe, Yutaka
通讯作者：
Takebe, Yutaka
影响因子：
5.5
作者：
Takebe, Yutaka;Liao, Huanan;Tee, Kok Keng
通讯作者：
Tee, Kok Keng
影响因子：
5.2
作者：
Lau, J. T. F.;Lin, C.;Gu, J.
通讯作者：
Gu, J.
影响因子：
39.2
作者：
Mellors, JW;Munoz, A;Rinaldo, CR
通讯作者：
Rinaldo, CR