The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation.
The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation.
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DOI：
10.1002/sctm.21-0264
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发表时间：
2021-12
影响因子：
6
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Liu C;Huang B;Wang H;Zhou J
血小板是止血的主要效应器，是由巨核细胞（MKs）产生的小无核血细胞，血小板产生或功能缺陷导致各种出血并发症。越来越多的证据表明，MK和血小板的多样性比以前所认识的要大得多，除了止血外，它们还参与许多生理和病理过程，如先天和适应性免疫反应、血管生成和肿瘤转移，而MK和血小板功能的个体变异也成为该领域的焦点。然而，mk和血小板是否通过利用不同的亚群来实现这些不同的功能仍然知之甚少。旨在在单细胞水平上破译MK转录组的新研究为MK的功能异质性提供了一些关键的见解。在这篇综述中，我们将讨论一些最近发现的mk的功能和发育异质性及其与血小板异质性的潜在联系。我们还将讨论这些发现的意义，同时关注人类多能干细胞在体外产生血小板。对人类MK发育和血小板产生的异质性的进一步了解将为未来血小板再生和相关疾病的临床治疗开辟新的途径。全面阐明巨核细胞（MK）异质性及其与血小板异质性的潜在联系，将为人类多能干细胞产生血小板提供新的见解。对于未来的临床应用，如具有止血、抗肿瘤或促血管生成功能的血小板偏倚mk和随后的具有不同功能的血小板亚群，将是极具吸引力的。
Platelets, the chief effector of hemostasis, are small anucleate blood cells generated from megakaryocytes (MKs), and the defects in platelet production or function lead to a variety of bleeding complications. Emerging evidence indicates that MKs and platelets are much more diverse than previously appreciated and involved in many physiological and pathological processes besides hemostasis, such as innate and adaptive immune responses, angiogenesis, and tumor metastasis, while the ontogenic variations in MK and platelet function have also become a focus in the field. However, whether MKs and platelets fulfill these distinct functions by utilizing distinct subpopulations remains poorly understood. New studies aimed at deciphering the MK transcriptome at the single‐cell level have provided some key insights into the functional heterogeneity of MKs. In this review, we will discuss some of the recent discoveries of functional and developmental heterogeneity of MKs and its potential link to the heterogeneity of platelets. We will also discuss the implications of these findings while focusing on the ex vivo generation of platelets from human pluripotent stem cells. The improved understanding of the heterogeneity underlying human MK development and platelet production should open new avenues for future platelet regeneration and clinical treatment of related diseases. The comprehensive elucidation of megakaryocyte (MK) heterogeneity and improved understanding of its potential link with platelet heterogeneity will provide new insights into the platelet production from human pluripotent stem cells. It would be extremely attractive to produce platelet‐biased MKs and subsequent platelet subpopulations with distinct functions for future clinical applications, such as those with hemostatic, antineoplastic, or pro‐angiogenic functions.
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DOI：
10.1083/jcb.201304054
发表时间：
2013-06-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Machlus KR;Italiano JE Jr
通讯作者：
Italiano JE Jr
人类巨核细胞的细胞异质性和免疫亚群的表征
DOI：
10.1002/advs.202100921
发表时间：
2021-08
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Liu C;Wu D;Xia M;Li M;Sun Z;Shen B;Liu Y;Jiang E;Wang H;Su P;Shi L;Xiao Z;Zhu X;Zhou W;Wang Q;Gao X;Cheng T;Zhou J
通讯作者：
Zhou J
影响因子：
7.7
作者：
Cunin, Pierre;Bouslama, Rim;Nigrovic, Peter A.
通讯作者：
Nigrovic, Peter A.
影响因子：
7.5
作者：
Battinelli, Elisabeth M.;Thon, Jonathan N.;Italiano, Joseph E., Jr.
通讯作者：
Italiano, Joseph E., Jr.
影响因子：
5.9
作者：
Feng, Qiang;Shabrani, Namrata;Thon, Jonathan N.;Huo, Hongguang;Thiel, Austin;Machlus, Kellie R.;Kim, Kyungho;Brooks, Julie;Li, Feng;Luo, Chenmei;Kimbrel, Erin A.;Wang, Jiwu;Kim, Kwang-Soo;Italiano, Joseph;Cho, Jaehyung;Lu, Shi-Jiang;Lanza, Robert
通讯作者：
Lanza, Robert