SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis.
SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis.
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SWAP 1-SFPS-RRC 1剪接因子复合物调节前体mRNA剪接促进拟南芥光形态建成
DOI：
10.1073/pnas.2214565119
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发表时间：
2022-11
影响因子：
11.1
通讯作者：
Huq, Enamul
中科院分区：
文献类型：
--
作者：
Kathare, Praveen Kumar;Xin, Ruijiao;Ganesan, Abirama Sundari;June, Viviana M.;Reddy, Anireddy S. N.;Huq, Enamul
转录调控和前信使RNA剪接是植物转录多样性和光调控基因表达的关键。虽然已经描述了几个转录因子，但只有几个剪接因子调节光信号通路。在这里，我们描述了剪接因子SWAP1的功能特性，它与两个先前描述的剪接因子SFP和RRC1相互作用，形成一个三元复合体。SWAP1还与光敏色素B(PhyB)相互作用。三个单、双突变组合在红光下表现出相似的下胚轴长度。SWAP1调节大量基因的选择性剪接，这些基因的一个子集受SFP、RRC1和SWAP1的协调调节。这些结果突显了PhyB相互作用的剪接因子在光调控植物发育中的重要性。由一组光感受器感知的光信号对植物的生理、生长和发育有着深远的影响。红/远红光吸收光敏色素(Phys)通过在多个水平上错综复杂地调控基因表达来调节这些方面。在这里，我们报告了一种RNA结合剪接因子SWAP1(白杏抑制因子/Surp RNA结合域包含蛋白1)的鉴定和功能鉴定。功能缺失的swap1-1突变体对红光不敏感，表现出与日长无关的早花表型。SWAP1与另外两个剪接因子相互作用，它们是光敏色素信号剪接因子(SFP)和CRY1CRY2背景1中红光反应减弱的剪接因子(RRC1)，它们以不依赖光的方式形成三元复合体。此外，SWAP1还与光活化的PhyB发生物理相互作用，并与核PhyB光体共定位。表型分析表明，swap1sfps、swap1rrc1和sfpsrrc1双突变体在红光下表现出与各自单突变体相似的下胚轴长度，表明它们在相同的遗传途径中发挥作用。Swap1sfps加倍和swap1sfpsrrc1三重突变体表现出多效性表型，包括成虫阶段的不育。深度RNA测序(RNA-seq)分析表明，SWAP1调控基因表达和大量基因的前信使RNA(MRNA)选择性剪接，包括那些与植物对光信号的反应有关的基因。对单个、双和三个突变体之间的选择性剪接的比较分析表明，所有这三个剪接因子都协调调节某一基因子集的选择性剪接。我们的研究揭示了一种剪接因子的功能，它通过与光激活的PhyB和其他剪接因子相互作用来调节光调节的选择性剪接。
Regulation of transcription and pre–messenger RNA (mRNA) splicing is essential for transcript diversity and light-regulated gene expression in plants. Although several transcription factors have been described, only a few splicing factors are known to regulate light signaling pathways. Here, we describe the functional characterization of splicing factor SWAP1, which interacts with two previously characterized splicing factors, SFPS and RRC1, forming a ternary complex. SWAP1 also interacts with photoactivated phytochrome B (phyB). All three single and double mutant combinations display similar hypocotyl lengths under red light. SWAP1 modulates alternative splicing of a large number of genes, and a subset of these genes is coordinately regulated by SFPS, RRC1, and SWAP1. These results highlight the importance of phyB-interacting splicing factors in light-regulated plant development. Light signals perceived by a group of photoreceptors have profound effects on the physiology, growth, and development of plants. The red/far-red light–absorbing phytochromes (phys) modulate these aspects by intricately regulating gene expression at multiple levels. Here, we report the identification and functional characterization of an RNA-binding splicing factor, SWAP1 (SUPPRESSOR-OF-WHITE-APRICOT/SURP RNA-BINDING DOMAIN-CONTAINING PROTEIN1). Loss-of-function swap1-1 mutant is hyposensitive to red light and exhibits a day length–independent early flowering phenotype. SWAP1 physically interacts with two other splicing factors, (SFPS) SPLICING FACTOR FOR PHYTOCHROME SIGNALING and (RRC1) REDUCED RED LIGHT RESPONSES IN CRY1CRY2 BACKGROUND 1 in a light-independent manner and forms a ternary complex. In addition, SWAP1 physically interacts with photoactivated phyB and colocalizes with nuclear phyB photobodies. Phenotypic analyses show that the swap1sfps, swap1rrc1, and sfpsrrc1 double mutants display hypocotyl lengths similar to that of the respective single mutants under red light, suggesting that they function in the same genetic pathway. The swap1sfps double and swap1sfpsrrc1 triple mutants display pleiotropic phenotypes, including sterility at the adult stage. Deep RNA sequencing (RNA-seq) analyses show that SWAP1 regulates the gene expression and pre–messenger RNA (mRNA) alternative splicing of a large number of genes, including those involved in plant responses to light signaling. A comparative analysis of alternative splicing among single, double, and triple mutants showed that all three splicing factors coordinately regulate the alternative splicing of a subset of genes. Our study uncovered the function of a splicing factor that modulates light-regulated alternative splicing by interacting with photoactivated phyB and other splicing factors.
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