Protocol for Drosophila sleep deprivation using single-chip board.
Protocol for Drosophila sleep deprivation using single-chip board.
复制标题
使用单芯片板的果蝇睡眠剥夺协议
DOI：
10.1016/j.xpro.2021.100827
复制
发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Han J
中科院分区：
文献类型：
--
作者：
Jin X;Gu P;Han J
睡眠行为的特点是长期静止和觉醒阈值增加，并且受到稳态调节。利用剥夺后的睡眠反弹来验证维持体内平衡的能力。该协议展示了如何建立一个编程的机械振荡系统和详细的程序来在果蝇中进行睡眠剥夺。该剥夺系统的特点是编程灵活性。完成该协议都需要电子电路知识和一定水平的编程。有关该协议的使用和执行的完整详细信息，请参阅。改进后的振荡器由伪随机触发信号控制可编程单芯片板可实现系统灵活性模块化设计简化了调试和维护睡眠行为的特点是长期静止和增加的唤醒阈值，并且它是稳态调节的。利用剥夺后的睡眠反弹来验证维持体内平衡的能力。该协议展示了如何建立一个编程的机械振荡系统和详细的程序来在果蝇中进行睡眠剥夺。该剥夺系统的特点是编程灵活性。完成该协议都需要电子电路知识和一定水平的编程。
Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in Drosophila. This deprivation system is featured by its programming flexibility. The knowledge of electronic circuits and a certain level of programming are both required to fulfill this protocol. For complete details on the use and execution of this protocol, please refer to. The retrofitted oscillator is controlled by a pseudo-random trigger signal A programmable single-chip board enables system flexibility The modular design simplifies debugging and maintenance Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in Drosophila. This deprivation system is featured by its programming flexibility. The knowledge of electronic circuits and a certain level of programming are both required to fulfill this protocol.
登录
查看更多内容
影响因子：
16.6
作者：
Robinson JE;Paluch J;Dickman DK;Joiner WJ
通讯作者：
Joiner WJ
影响因子：
3.7
作者：
Donelson NC;Kim EZ;Slawson JB;Vecsey CG;Huber R;Griffith LC
通讯作者：
Griffith LC
影响因子：
1.9
作者：
Anagnostou, Christiana;Dorsch, Monika;Rohlfs, Marko
通讯作者：
Rohlfs, Marko
影响因子：
2.7
作者：
GANTER, PF
通讯作者：
GANTER, PF
DN1p 神经元的一个子集整合热感觉输入，通过 CNMa 信号传导促进觉醒
影响因子：
9.2
作者：
Jin, Xi;Tian, Yao;Han, Junhai
通讯作者：
Han, Junhai