Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis.
Role of the luxS gene in bacteriocin biosynthesis by Lactobacillus plantarum KLDS1.0391: A proteomic analysis.
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luxS 基因在植物乳杆菌 KLDS1.0391 细菌素生物合成中的作用：蛋白质组分析
DOI：
10.1038/s41598-017-13231-4
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发表时间：
2017-10-24
影响因子：
4.6
通讯作者：
Meng XC
中科院分区：
文献类型：
--
作者：
Jia FF;Pang XH;Zhu DQ;Zhu ZT;Sun SR;Meng XC
某些乳酸菌的益生菌，特别是植物乳杆菌，通过群体感应(QS)系统调节细菌素的合成。本研究旨在探讨在植物乳杆菌KLDS1.0391细菌素合成过程中，LuxS介导的QS的分子机制。在没有LUX S的情况下，草坪斑点法表明，植物乳杆菌KLDS1.0391与瑞士乳杆菌KLDS1.9207共培养时细菌素产量显著降低(P0.01)，而单独培养时细菌素产量无显著变化。此外，高效液相色谱-电喷雾电离串联质谱仪分析表明，植物乳杆菌KLDS1.0391作为对LuxS缺失的响应，改变了与碳水化合物代谢、氨基酸代谢、脂肪酸合成和代谢以及双组分调控系统相关的蛋白质的表达水平。特别是，在共培养过程中，来自双组分系统LyttR家族的感受器组氨酸蛋白激酶AgC在LuxS突变体和野生型菌株中的表达存在差异，而与细菌素生物合成相关的蛋白质在单独培养时没有发现显著差异。综上所述，我们发现细菌素的产生受到碳水化合物代谢、氨基酸代谢、脂肪酸合成和代谢以及双组分调控系统的调节。此外，我们的结果还证明了LuxS介导的分子机制在细菌素生产中的作用。
Certain probiotic species of lactic acid bacteria, especially Lactobacillus plantarum, regulate bacteriocin synthesis through quorum sensing (QS) systems. In this study, we aimed to investigate the luxS-mediated molecular mechanisms of QS during bacteriocin synthesis by L. plantarum KLDS1.0391. In the absence of luxS, the ‘spot-on-the-lawn’ method showed that the bacteriocin production by L. plantarum KLDS1.0391 significantly decreased upon co-cultivation with L. helveticus KLDS1.9207 (P 0.01) but did not change significantly when mono-cultivated. Furthermore, liquid chromatography-electrospray ionization tandem mass spectrometry analysis showed that, as a response to luxS deletion, L. plantarum KLDS1.0391 altered the expression level of proteins involved in carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. In particular, the sensor histidine kinase AgrC (from the two-component system, LytTR family) was expressed differently between the luxS mutant and the wild-type strain during co-cultivation, whereas no significant differences in proteins related to bacteriocin biosynthesis were found upon mono-cultivation. In summary, we found that the production of bacteriocin was regulated by carbohydrate metabolism, amino acid metabolism, fatty acid synthesis and metabolism, and the two-component regulatory system. Furthermore, our results demonstrate the role of luxS-mediated molecular mechanisms in bacteriocin production.
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