Simultaneous Determination of Columbianadin and Its Metabolite Columbianetin in Rat Plasma by LC-MS/MS: Application to Pharmacokinetics of Columbianadin after Oral Administration.
Simultaneous Determination of Columbianadin and Its Metabolite Columbianetin in Rat Plasma by LC-MS/MS: Application to Pharmacokinetics of Columbianadin after Oral Administration.
复制标题
LC-MS/MS 同时测定大鼠血浆中 Columbianadin 及其代谢物 Columbianetin：在口服给药后 Columbianadin 药代动力学中的应用
DOI：
10.1155/2018/8568303
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chang YX
中科院分区：
文献类型：
--
作者：
Li J;Li Z;Luo Q;Wang CP;He J;Pang X;Teye Azietaku J;Chang YX
Columbianadin及其代谢产物Columbianadin具有抗炎、镇痛、钙通道阻滞和抗肿瘤活性。为比较大鼠口服纯品Columbianadin及其代谢产物Columbianoprotein后的药代动力学差异，建立了一种简便、灵敏的液相色谱-串联质谱（LC-MS/MS）法同时测定大鼠血浆中Columbianadin和Columbianoprotein浓度。两种分析物和内标物（华法林）分离和测定后，用乙酸乙酯液-液萃取。以乙酸铵水溶液（1 mmol/L）和乙腈为移动的相，流速为0.3 mL/min，定量下限（LLOQ）分别为0.1 ng/mL和0.5 ng/mL。单剂量口服Columbianadin纯品和APR提取物后，Columbianadin的一些药代动力学参数和生物利用度之间存在显著差异。开展比较药代动力学研究，对于促进Columbianadin的临床应用和开发潜在的APR具有重要意义。
Columbianadin and its metabolite columbianetin exhibited the anti-inflammatory, analgesic, calcium channel blocking and antitumor activities. To compare the differences between pharmacokinetics of columbianadin and its metabolite columbianetin after oral administration of pure columbianadin and Angelicae Pubescentis Radix (APR) extract, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established and validated to simultaneously determine columbianadin and columbianetin in rat plasma. Two analytes and an internal standard (warfarin) were well separated and determined after liquid-liquid extraction with ethyl acetate. Ammonium acetate aqueous solution (1 mmol/L) and acetonitrile were used as the mobile phase and the flow rate was 0.3 mL/min. The lower limit of quantification (LLOQ) was 0.1 ng/mL for columbianetin and 0.5 ng/mL for columbianadin, respectively. There were significant differences between some pharmacokinetic parameters and bioavailability of columbianadin after oral administration of pure columbianadin and APR extract. The studies on comparative pharmacokinetics of columbianadin were of great use for facilitating the clinical application of columbianadin and were also highly meaningful for the potential development of APR.
登录
查看更多内容
DOI：
10.3390/molecules200711719
发表时间：
2015-06-25
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Yang YF;Xu W;Song W;Ye M;Yang XW
通讯作者：
Yang XW
影响因子：
5.4
作者：
Luo, Qian;Wang, Chun-peng;Chang, Yan-xu
通讯作者：
Chang, Yan-xu
影响因子：
1.8
作者：
Zhang, You-Bo;Yang, Xiu-Wei
通讯作者：
Yang, Xiu-Wei
影响因子：
3.4
作者：
Bahadir, Ozlem;Citoglu, Gulcin Saltan;Smejkal, Karel
通讯作者：
Smejkal, Karel
DOI：
10.3736/jcim20080413
发表时间：
2008-04-01
期刊：
Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
影响因子：
--
作者：
Yang, Xiu-wei;Guo, Qing-mei;Wang, Ying
通讯作者：
Wang, Ying