TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells.
TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells.
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TRPM5是一种味觉信号的瞬态受体电位离子通道，是化学感应细胞中普遍存在的信号传导成分。
DOI：
10.1186/1471-2202-8-49
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发表时间：
2007-07-04
期刊：
影响因子：
2.4
通讯作者：
Chubanov, Vladimir
中科院分区：
文献类型：
--
作者：
Kaske, Silke;Krasteva, Gabriele;Koenig, Peter;Kummer, Wolfgang;Hofmann, Thomas;Gudermann, Thomas;Chubanov, Vladimir
越来越多的TRP通道已被确定为嗅觉，温度，机械力和味觉感觉的关键参与者。已知TRPM 5在味觉受体细胞中大量表达，在味觉受体细胞中TRPM 5参与甜味、氨基酸和苦味的感知。然而，TRPM 5在其他感觉系统中的作用迄今尚未研究。在这里，我们系统地研究了TRPM 5在大鼠和小鼠组织中的表达。除了味蕾，我们发现TRPM 5主要定位于味觉受体细胞的基底外侧表面，TRPM 5免疫反应性在其他化学感觉器官-主要的嗅觉上皮和犁鼻器。最引人注目的是，我们在呼吸道和胃肠道的所有部分都发现了孤立的TRPM 5富集的上皮细胞。基于它们的组织分布、低细胞密度、形态学特征和与不同上皮标记物的共免疫染色，我们将这些细胞鉴定为刷状细胞（也称为簇状细胞、纤维泡状细胞、多泡状细胞或小窝状细胞）。刷状细胞的形态特征与味觉感受器细胞相似，但其起源和生物学作用仍存在争议。我们认为TRPM 5是哺乳动物化学感受器官的内在信号成分，并为刷状细胞在外周中是重要的细胞相关物提供证据。
A growing number of TRP channels have been identified as key players in the sensation of smell, temperature, mechanical forces and taste. TRPM5 is known to be abundantly expressed in taste receptor cells where it participates in sweet, amino acid and bitter perception. A role of TRPM5 in other sensory systems, however, has not been studied so far. Here, we systematically investigated the expression of TRPM5 in rat and mouse tissues. Apart from taste buds, where we found TRPM5 to be predominantly localized on the basolateral surface of taste receptor cells, TRPM5 immunoreactivity was seen in other chemosensory organs – the main olfactory epithelium and the vomeronasal organ. Most strikingly, we found solitary TRPM5-enriched epithelial cells in all parts of the respiratory and gastrointestinal tract. Based on their tissue distribution, the low cell density, morphological features and co-immunostaining with different epithelial markers, we identified these cells as brush cells (also known as tuft, fibrillovesicular, multivesicular or caveolated cells). In terms of morphological characteristics, brush cells resemble taste receptor cells, while their origin and biological role are still under intensive debate. We consider TRPM5 to be an intrinsic signaling component of mammalian chemosensory organs, and provide evidence for brush cells being an important cellular correlate in the periphery.
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影响因子：
64.5
作者：
Adler, E;Hoon, MA;Zuker, CS
通讯作者：
Zuker, CS
影响因子：
2.5
作者：
Clapp, TR;Yang, RB;Kinnamon, JC
通讯作者：
Kinnamon, JC
DOI：
10.1679/aohc1950.41.87
发表时间：
1978-01-01
期刊：
ARCHIVUM HISTOLOGICUM JAPONICUM
影响因子：
--
作者：
AKISAKA, T;ODA, M
通讯作者：
ODA, M
DOI：
10.1023/a:1026548020851
发表时间：
2000-03-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Höfer, D;Shin, DW;Drenckhahn, D
通讯作者：
Drenckhahn, D
影响因子：
64.5
作者：
Chandrashekar, J;Mueller, KL;Ryba, NJP
通讯作者：
Ryba, NJP