Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease.
Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease.
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主动脉瘤和闭塞性疾病中纤溶酶原激活剂的异常表达。
DOI：
10.1016/s0741-5214(94)70012-5
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发表时间：
1994
影响因子：
4.3
通讯作者：
Lucore,CL
中科院分区：
文献类型：
--
作者：
Reilly,JM;Sicard,GA;Lucore,CL
目的和方法：主动脉瘤的特征是介质细胞外基质的破坏，而闭塞性疾病则涉及内膜内过量基质的积累。纤溶蛋白通过激活潜在的金属酶直接或间接地降解细胞外基质。为了确定组织型和尿激酶型纤溶酶原激活剂的表达，对动脉瘤（n= 12）、闭塞（n= 8）和健康（n= 6）主动脉标本进行了免疫测定、纤维蛋白自显影、Northern分析和免疫组织化学。结果：组织型纤溶酶原激活剂免疫分析显示，动脉瘤主动脉组织型纤溶酶原激活剂提取物蛋白含量为8.7±0.9 ng/mg，正常主动脉为5.7±0.3 ng/mg，闭塞主动脉为2.5±0.3 ng/mg（各组比较p< 0.05）。尿激酶型纤溶酶原激活物免疫分析法未检出尿激酶型纤溶酶原激活物抗原。纤维蛋白自显影显示64 kDa和54 kDa的溶酶活性，这归因于组织型纤溶酶原激活剂和尿激酶型纤溶酶原激活剂。绝大多数纤维蛋白溶解继发于游离组织型纤溶酶原激活剂，在动脉瘤性疾病中最多，在闭塞性疾病中最少。继发于尿激酶型纤溶酶原激活剂的酶解较少。组织型纤溶酶原激活物和尿激酶型纤溶酶原激活物mRNA的表达在动脉瘤性和闭塞性主动脉中具有可比性。与闭塞性疾病不同，动脉瘤有炎性细胞浸润，其特征是特异性单核细胞表达尿激酶型纤溶酶原激活物。组织型纤溶酶原激活物在正常、病变主动脉内膜及病变主动脉中均有明显表达。结论：纤溶酶原激活剂在动脉壁内的差异表达可能是动脉瘤性和闭塞性主动脉疾病独特的发病机制之一。(J VASCSURG1994; 19:865 - 72)
Purpose and Methods: Aortic aneurysms are characterized by the destruction of the extracellular matrix of the media, whereas occlusive disease involves excess matrix accumulation within the intima. Plasmin degrades extracellular matrix directly and indirectly by activation of latent metalloenzymes. To determine the expression of tissue- and urokinase-type plasminogen activators, immunoassay, fibrin autography, Northern analysis, and immunohistochemistry were performed on specimens of aneurysmal (n= 12), occlusive (n= 8), and healthy (n= 6) aorta.Results:Immunoassay of tissue-type plasminogen activator revealed 8.7 ± 0.9 ng tissue-type plasminogen activator/mg extracted protein in aneurysmal aorta, 5.7 ± 0.3 ng/mg in normal aorta, and 2.5 ± 0.3 ng/mg in occlusive aorta (p< 0.05 for comparisons between all groups). No urokinase-type plasminogen activator antigen was detected by urokinase-type plasminogen activator immunoassay. Fibrin autography exhibited lytic activity at 64 kDa and 54 kDa attributable to tissue-type plasminogen activator and urokinase-type plasminogen activator. The vast majority of fibrinolysis was secondary to free tissue-type plasminogen activator and was greatest in aneurysmal disease and least in occlusive disease. There was only a small amount of lysis secondary to urokinase-type plasminogen activator. Expression of tissue-type plasminogen activator and urokinase-type plasminogen activators mRNA was comparable in aneurysmal and occlusive aortas. In contrast to occlusive disease, aneurysms had an inflammatory cell infiltrate characterized by the expression of urokinase-type plasminogen activator by specific mononuclear cells. Tissue-type plasminogen activator expression was evident in the intima of normal and diseased aorta and in the media of diseased aorta.Conclusion: Differential expression of plasminogen activators within the arterial wall may contribute to the unique pathogenesis of aneurysmal and occlusive aortic disease. (J VASCSURG1994;19:865-72)
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影响因子：
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作者：
Lucore,CL;Fujii,S;Sobel,BE
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影响因子：
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