Genetic and environmental controls on nitrous oxide accumulation in lakes.
Genetic and environmental controls on nitrous oxide accumulation in lakes.
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湖泊中一氧化二氮积累的遗传和环境控制。
DOI：
10.1371/journal.pone.0121201
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tiirola M
中科院分区：
文献类型：
--
作者：
Saarenheimo J;Rissanen AJ;Arvola L;Nykänen H;Lehmann MF;Tiirola M
研究了12个北方湖泊中环境因素、氧化亚氮（N2O）积累与亚硝酸盐和N2O还原菌遗传指标之间的潜在联系。通过对湖泊沉积物中亚硝酸盐还原酶和N2O还原酶编码基因（nirS/nirK和nosZ）的定量研究，研究了湖泊沉积物中反硝化细菌，包括两个系统发育不同的分支nosZ I和nosZ II。在湖际尺度和单个湖泊（Vanajavesi湖）的深度样带内，夏季N2O积累与低通量硝酸盐浓度均呈正相关。各湖泊nirS、nirK、nosZ I和nosZ II基因丰度的变异较高（高达10倍），这使我们能够研究生态系统nir-vs-nos基因清单与N2O积累之间的预期联系。湖泊间N2O积累的变化确实与亚硝酸盐与N2O还原酶基因的相对丰度有关，即(nirS+nirK)/nosZ I基因比。此外，湖际尺度上的(nirS+nirK)/nosZ I和湖内的(nirS+nirK)/nosZ I+II与硝态氮有效性呈正相关。结果表明，环境硝酸盐浓度可能是湖泊生态系统N2O积累的重要调节因子，其直接方式是通过增加总体反硝化速率，间接方式是通过控制亚硝酸盐与N2O还原酶携带生物的平衡。
We studied potential links between environmental factors, nitrous oxide (N2O) accumulation, and genetic indicators of nitrite and N2O reducing bacteria in 12 boreal lakes. Denitrifying bacteria were investigated by quantifying genes encoding nitrite and N2O reductases (nirS/nirK and nosZ, respectively, including the two phylogenetically distinct clades nosZ I and nosZ II) in lake sediments. Summertime N2O accumulation and hypolimnetic nitrate concentrations were positively correlated both at the inter-lake scale and within a depth transect of an individual lake (Lake Vanajavesi). The variability in the individual nirS, nirK, nosZ I, and nosZ II gene abundances was high (up to tenfold) among the lakes, which allowed us to study the expected links between the ecosystem’s nir-vs-nos gene inventories and N2O accumulation. Inter-lake variation in N2O accumulation was indeed connected to the relative abundance of nitrite versus N2O reductase genes, i.e. the (nirS+nirK)/nosZ I gene ratio. In addition, the ratios of (nirS+nirK)/nosZ I at the inter-lake scale and (nirS+nirK)/nosZ I+II within Lake Vanajavesi correlated positively with nitrate availability. The results suggest that ambient nitrate concentration can be an important modulator of the N2O accumulation in lake ecosystems, either directly by increasing the overall rate of denitrification or indirectly by controlling the balance of nitrite versus N2O reductase carrying organisms.
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影响因子：
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