Catalog of Differentially Expressed Long Non-Coding RNA following Activation of Human and Mouse Innate Immune Response.
Catalog of Differentially Expressed Long Non-Coding RNA following Activation of Human and Mouse Innate Immune Response.
复制标题
DOI：
10.3389/fimmu.2017.01038
复制
发表时间：
2017
影响因子：
7.3
通讯作者：
Lindsay MA
中科院分区：
文献类型：
--
作者：
Roux BT;Heward JA;Donnelly LE;Jones SW;Lindsay MA
尽管越来越多的证据表明长链非编码rna （lncRNAs）是一种新的免疫调节因子，但目前还没有系统的尝试来鉴定和表征在诱导先天免疫反应后表达改变的lncRNAs。为了解决这个问题，我们利用下一代测序数据来确定四种人类细胞（单核细胞、巨噬细胞、上皮细胞和软骨细胞）和四种小鼠细胞类型（RAW 264.7巨噬细胞、骨髓源性巨噬细胞、腹膜巨噬细胞和脾树突状细胞）暴露于促炎介质、脂多糖（LPS）或白细胞介素-1β后lncRNA谱的变化。我们发现了204个人类lncrna和210个小鼠lncrna的差异表达，定位分析显示与免疫相关基因相关。这些lncrna主要是细胞类型特异性的，由大区域的重复序列组成，并且表现出较差的进化保守性。虽然我们发现了多个保守基序，但人类和小鼠序列的比较显示不到1%的序列保守性。在204个人类lncrna中，21个与syntenic小鼠lncrna重叠，其中5个在两种物种中表达差异。在这些合成lncRNA中有IL7-AS（反义），它在多种细胞类型中被诱导，并在人和小鼠细胞中调节促炎介质白细胞介素-6的产生。综上所述，我们已经鉴定并表征了人类和小鼠先天免疫反应激活后差异表达的lncrna，相信这些目录将为未来分析lncrna在免疫和炎症反应中的作用提供基础。
Despite increasing evidence to indicate that long non-coding RNAs (lncRNAs) are novel regulators of immunity, there has been no systematic attempt to identify and characterize the lncRNAs whose expression is changed following the induction of the innate immune response. To address this issue, we have employed next-generation sequencing data to determine the changes in the lncRNA profile in four human (monocytes, macrophages, epithelium, and chondrocytes) and four mouse cell types (RAW 264.7 macrophages, bone marrow-derived macrophages, peritoneal macrophages, and splenic dendritic cells) following exposure to the pro-inflammatory mediators, lipopolysaccharides (LPS), or interleukin-1β. We show differential expression of 204 human and 210 mouse lncRNAs, with positional analysis demonstrating correlation with immune-related genes. These lncRNAs are predominantly cell-type specific, composed of large regions of repeat sequences, and show poor evolutionary conservation. Comparison within the human and mouse sequences showed less than 1% sequence conservation, although we identified multiple conserved motifs. Of the 204 human lncRNAs, 21 overlapped with syntenic mouse lncRNAs, of which five were differentially expressed in both species. Among these syntenic lncRNA was IL7-AS (antisense), which was induced in multiple cell types and shown to regulate the production of the pro-inflammatory mediator interleukin-6 in both human and mouse cells. In summary, we have identified and characterized those lncRNAs that are differentially expressed following activation of the human and mouse innate immune responses and believe that these catalogs will provide the foundation for the future analysis of the role of lncRNAs in immune and inflammatory responses.
登录
查看更多内容
影响因子：
5.8
作者：
Barnett, Derek W.;Garrison, Erik K.;Marth, Gabor T.
通讯作者：
Marth, Gabor T.
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
影响因子：
29.7
作者：
Brubaker SW;Bonham KS;Zanoni I;Kagan JC
通讯作者：
Kagan JC
影响因子：
30.5
作者：
Casero D;Sandoval S;Seet CS;Scholes J;Zhu Y;Ha VL;Luong A;Parekh C;Crooks GM
通讯作者：
Crooks GM