Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish.
Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish.
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Duox是斑马鱼成年尾鳍再生过程中负责ROS产生的主要NADPH氧化酶。
DOI：
10.1016/j.isci.2023.106147
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发表时间：
2023-03-17
期刊：
影响因子：
5.8
通讯作者：
Amaya, Enrique
中科院分区：
文献类型：
--
作者：
Chopra, Kunal;Folkmanait, Milda;Stockdale, Liam;Shathish, Vishali;Ishibashi, Shoko;Bergin, Rachel;Amich, Jorge;Amaya, Enrique
活性氧（ROS）水平的持续升高已被证明是许多生物体再生所必需的。这主要通过使用靶向NADPH氧化酶（NOX）家族的药理学抑制剂来证明。为了鉴定斑马鱼成年尾鳍再生过程中参与ROS产生的特定NOX，我们产生了duox，nox 5和cyba（NOX 1-4的关键亚基）的NOX突变体，并将这些品系与普遍表达HyPer的转基因品系杂交，从而可以测量ROS水平。纯合duox突变体中的单突变体的活性氧水平和鳍再生率的影响最大。然而，duox：cyba双突变体表现出更大的影响，鳍再生比单一duox突变体，这表明Nox 1 -4也发挥了作用，在再生过程中。这项工作还意外地发现，截肢的成年斑马鱼鳍中的ROS水平以昼夜节律振荡。断鳍导致ROS升高，与生物钟一起振荡duox突变体在断鳍后ROS水平降低duox突变体尾鳍再生率降低duox/cyba突变体比duox突变体在再生方面有更严重的缺陷细胞生物学;鱼类学;分子遗传学
Sustained elevated levels of reactive oxygen species (ROS) have been shown to be essential for regeneration in many organisms. This has been shown primarily via the use of pharmacological inhibitors targeting the family of NADPH oxidases (NOXes). To identify the specific NOXes involved in ROS production during adult caudal fin regeneration in zebrafish, we generated nox mutants for duox, nox5 and cyba (a key subunit of NOXes 1–4) and crossed these lines with a transgenic line ubiquitously expressing HyPer, which permits the measurement of ROS levels. Homozygous duox mutants had the greatest effect on ROS levels and rate of fin regeneration among the single mutants. However, duox:cyba double mutants showed a greater effect on fin regeneration than the single duox mutants, suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm. Fin amputation results in elevated ROS that oscillate with the circadian clock duox mutants have diminished ROS levels following caudal fin amputation duox mutants have diminished rate of caudal fin regeneration duox/cyba mutants have a more severe defect in regeneration than duox mutants Cell biology; Ichthyology; Molecular genetics
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