Nanohole arrays of mixed designs and microwriting for simultaneous and multiple protein binding studies.
Nanohole arrays of mixed designs and microwriting for simultaneous and multiple protein binding studies.
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DOI：
10.1016/j.bios.2009.02.017
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发表时间：
2009-05-15
影响因子：
12.6
通讯作者：
Larson DN
中科院分区：
文献类型：
--
作者：
Ji J;Yang JC;Larson DN
我们演示了使用混合设计的纳米孔阵列和基于浸笔纳米光刻的微写入过程，根据纳米孔阵列的非凡光传输强度同时实时监测多个不同的蛋白质结合事件。微写入过程和单个纳米孔阵列的小足迹使我们能够观察相距仅 16μm 的不同结合事件，从而实现高空间分辨率。我们还提出了一种新颖的概念，该概念结合了不同设计的纳米孔阵列，以提高结合研究的置信度和准确性。为了证明概念，在一个传感器上制造了两种类型的纳米孔阵列，旨在对蛋白质结合表现出相反的响应。初步研究表明，混合设计可以帮助筛选出蛋白质内在信号等伪影，从而提高结合解释的准确性。
We demonstrate using nanohole arrays of mixed designs and a microwriting process based on dip-pen nanolithography to monitor multiple, different protein binding events simultaneously in real time based on the intensity of Extraordinary Optical Transmission of nanohole arrays. The microwriting process and small footprint of the individual nanohole arrays enabled us to observe different binding events located only 16μm apart, achieving high spatial resolution. We also present a novel concept that incorporates nanohole arrays of different designs to improve confidence and accuracy of binding studies. For proof of concept, two types of nanohole arrays, designed to exhibit opposite responses to protein bindings, were fabricated on one transducer. Initial studies indicate that the mixed designs could help to screen out artifacts such as protein intrinsic signals, providing improved accuracy of binding interpretation.
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影响因子：
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作者：
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通讯作者：
Mirkin, CA
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作者：
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