Claudin-2-dependent changes in noncharged solute flux are mediated by the extracellular domains and require attachment to the PDZ-scaffold.
Claudin-2-dependent changes in noncharged solute flux are mediated by the extracellular domains and require attachment to the PDZ-scaffold.
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DOI：
10.1111/j.1749-6632.2009.04052.x
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发表时间：
2009-05
影响因子：
5.2
通讯作者：
Anderson JM
中科院分区：
文献类型：
--
作者：
Van Itallie CM;Holmes J;Bridges A;Anderson JM
通过紧密连接的细胞旁路运输显示出对离子电荷和溶质大小的选择性。已知紧密蛋白胞外环上的带电残基控制电荷选择性。还已知claudin-2而非claudin-4的诱导表达将选择性地增加半径<4Å的PEG分子的渗透性，但尚不清楚渗透性是否由控制电荷选择性的claudin的相同区域控制。利用 MDCK II 细胞单层中 Claudin-2 和 Claudin-4 嵌合体的诱导表达，我们发现细胞外环单独负责控制不带电 PEG 的渗透性以及电荷选择性。此外，野生型claudin-2需要细胞质C端PDZ结合基序来控制渗透性，这表明需要附着到PDZ支架上才能形成孔。这些观察结果支持一个模型，其中环形成孔，控制小于 4Å 的带电和不带电溶质的渗透性。他们没有回答为什么claudin-2和-4都可以影响电性能，而只有-2可以选择性地增加小PEG的渗透性。
Paracellular transport through the tight junction shows selectivity for both ionic charge and solute size. It is known that charged residues on the extracellular loops of claudins control charge selectivity. It is also known that inducible expression of claudin-2, but not claudin-4, will selectively increase the permeability for PEG molecules which are <4Å in radius, but it is not known whether permeability is controlled by the same regions of claudins which control charge selectivity. Using inducible expression of chimeras of claudin-2 and claudin-4 in monolayers of MDCK II cells we show that the extracellular loops alone are responsible for controlling the permeability for noncharged PEGs as well as for charge selectivity. Further, the cytoplasmic C-terminal PDZ-binding motif is required for wild type claudin-2 to control permeability, suggesting a requirement for attachment to the PDZ scaffold in order to form pores. These observations support a model where the loops form pores controlling permeability for both charged and noncharged solutes which are smaller than 4Å. They leave unanswered why both claudin-2 and -4 can influence electrical properties while only -2 can selectively increase permeability for small PEGs.
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影响因子：
5.5
作者：
Colegio, OR;Van Itallie, C;Anderson, JM
通讯作者：
Anderson, JM
影响因子：
3.7
作者：
Tavelin, S;Taipalensuu, J;Artursson, P
通讯作者：
Artursson, P
DOI：
10.1152/ajpcell.2001.281.2.c388
发表时间：
2001-08-01
影响因子：
5.5
作者：
Watson, CJ;Rowland, M;Warhurst, G
通讯作者：
Warhurst, G
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影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Tsukita, S
通讯作者：
Tsukita, S
影响因子：
3.2
作者：
Ridyard, Alison E.;Brown, Jeremy K.;Miller, Hugh R. P.
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Miller, Hugh R. P.